Synthesis of hemostatic aerogel of TEMPO-oxidized cellulose nanofibers/collagen/chitosan and in vivo/vitro evaluation

Lu Liu; Liang Liu; Lin Chen; Genqiang Chen; Yen Wei; Feng F. Hong

Materials Today Bio (Oct 2024)

Synthesis of hemostatic aerogel of TEMPO-oxidized cellulose nanofibers/collagen/chitosan and in vivo/vitro evaluation

Lu Liu,
Liang Liu,
Lin Chen,
Genqiang Chen,
Yen Wei,
Feng F. Hong

Affiliations

Lu Liu: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, 201620, China; College of Biological Science and Medical Engineering, Donghua University, No.2999 North Ren Min Road, Shanghai, 201620, China
Liang Liu: College of Biological Science and Medical Engineering, Donghua University, No.2999 North Ren Min Road, Shanghai, 201620, China
Lin Chen: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, 201620, China
Genqiang Chen: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, 201620, China; College of Biological Science and Medical Engineering, Donghua University, No.2999 North Ren Min Road, Shanghai, 201620, China; Corresponding author. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, 201620, China.
Yen Wei: Department of Chemistry and the Tsinghua Center for Frontier Polymer Research, Tsinghua University, Beijing, 100084, China
Feng F. Hong: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, 201620, China; College of Biological Science and Medical Engineering, Donghua University, No.2999 North Ren Min Road, Shanghai, 201620, China; National Advanced Functional Fiber Innovation Center, WuJiang, Suzhou, China; Scientific Research Base for Bacterial Nanofiber Manufacturing and Composite Technology, China Textile Engineering Society, Shanghai, 201620, China; Corresponding author. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, 201620, China.

Journal volume & issue: Vol. 28
p. 101204

Abstract

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The treatment of internal hemorrhage remains challenging due to the current limited antibacterial capability, hemostatic efficacy, and biocompatibility of hemostatic materials. The TEMPO-oxidized cellulose nanofibers/collagen/chitosan (TCNF/COL/CS) hemostatic aerogel was developed in this work by physically encasing COL in a sandwich structure and electrostatically self-assembling polyanionic TCNF with polycationic CS. In vitro coagulation experiments revealed the favorable procoagulant properties of TCNF/COL/CS along with high adhesion to erythrocytes and platelets. TCNF/COL/CS significantly increased the hemostatic efficacy by 59.8 % and decreased blood loss by 62.2 % in the liver injury model when compared to Surgicel®, the most frequently used hemostatic material. Furthermore, it demonstrated outstanding biodegradability both in vitro and in vivo, and a substantial increase in resistance (96.8 % against E. coli and 95.4 % against S. aureus) compared to TCNF. The significant hemostatic and biodegradable characteristics of TCNF/COL/CS can be ascribed to its interconnected porous structure, increased porosity, and efficient water absorption, along with the synergistic effect of the three constituents. The TCNF/COL/CS aerogel shows significant potential to control internal bleeding. A novel plant-derived nanocellulose composite aerogel has been described here for the first time; it has outstanding antibacterial characteristics, higher biocompatibility, and outstanding hemostatic characteristics in vivo.

Published in Materials Today Bio

ISSN: 2590-0064 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Biology (General)
Website: https://www.journals.elsevier.com/materials-today-bio

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