Antimicrobial Contribution of Chitosan Surface-Modified Nanoliposomes Combined with Colistin against Sensitive and Colistin-Resistant Clinical <i>Pseudomonas aeruginosa</i>
Valentina Laverde-Rojas,
Yamil Liscano,
Sandra Patricia Rivera-Sánchez,
Ivan Darío Ocampo-Ibáñez,
Yeiston Betancourt,
Maria José Alhajj,
Cristhian J. Yarce,
Constain H. Salamanca,
Jose Oñate-Garzón
Affiliations
Valentina Laverde-Rojas
Facultad de Salud, Programa de Medicina, Universidad Santiago de Cali, Calle 5 No. 62-00, Cali 760035, Colombia
Yamil Liscano
Facultad de Ciencias Básicas, Programa de Microbiología, Universidad Santiago de Cali, Calle 5 No. 62-00, Cali 760035, Colombia
Sandra Patricia Rivera-Sánchez
Facultad de Ciencias Básicas, Programa de Microbiología, Universidad Santiago de Cali, Calle 5 No. 62-00, Cali 760035, Colombia
Ivan Darío Ocampo-Ibáñez
Facultad de Ciencias Básicas, Programa de Microbiología, Universidad Santiago de Cali, Calle 5 No. 62-00, Cali 760035, Colombia
Yeiston Betancourt
Facultad de Ciencias Básicas, Programa de Microbiología, Universidad Santiago de Cali, Calle 5 No. 62-00, Cali 760035, Colombia
Maria José Alhajj
Laboratorio de Diseño y Formulación de Productos Químicos y Derivados, Departamento de Ciencias Farmacéuticas, Facultad de Ciencias Naturales, Universidad Icesi, Cali 760035, Colombia
Cristhian J. Yarce
Laboratorio de Diseño y Formulación de Productos Químicos y Derivados, Departamento de Ciencias Farmacéuticas, Facultad de Ciencias Naturales, Universidad Icesi, Cali 760035, Colombia
Constain H. Salamanca
Laboratorio de Diseño y Formulación de Productos Químicos y Derivados, Departamento de Ciencias Farmacéuticas, Facultad de Ciencias Naturales, Universidad Icesi, Cali 760035, Colombia
Jose Oñate-Garzón
Facultad de Ciencias Básicas, Programa de Microbiología, Universidad Santiago de Cali, Calle 5 No. 62-00, Cali 760035, Colombia
Colistin is a re-emergent antibiotic peptide used as a last resort in clinical practice to overcome multi-drug resistant (MDR) Gram-negative bacterial infections. Unfortunately, the dissemination of colistin-resistant strains has increased in recent years and is considered a public health problem worldwide. Strategies to reduce resistance to antibiotics such as nanotechnology have been applied successfully. In this work, colistin was characterized physicochemically by surface tension measurements. Subsequently, nanoliposomes coated with highly deacetylated chitosan were prepared with and without colistin. The nanoliposomes were characterized using dynamic light scattering and zeta potential measurements. Both physicochemical parameters fluctuated relatively to the addition of colistin and/or polymer. The antimicrobial activity of formulations increased by four-fold against clinical isolates of susceptible Pseudomona aeruginosa but did not have antimicrobial activity against multidrug-resistant (MDR) bacteria. Interestingly, the free coated nanoliposomes exhibited the same antibacterial activity in both sensitive and MDR strains. Finally, the interaction of colistin with phospholipids was characterized using molecular dynamics (MD) simulations and determined that colistin is weakly associated with micelles constituted by zwitterionic phospholipids.