Frontiers in Microbiology (Sep 2022)

Metagenomic analysis of the soil microbial composition and salt tolerance mechanism in Yuncheng Salt Lake, Shanxi Province

  • Feifeng Zeng,
  • Yonghong Zhu,
  • Dongling Zhang,
  • Zengqiang Zhao,
  • Quansheng Li,
  • Panpan Ma,
  • Guoli Zhang,
  • Yuan Wang,
  • Shenjie Wu,
  • Sandui Guo,
  • Guoqing Sun

DOI
https://doi.org/10.3389/fmicb.2022.1004556
Journal volume & issue
Vol. 13

Abstract

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The soil in Yuncheng Salt Lake has serious salinization and the biogeographic environment affects the composition and distribution of special halophilic and salt-tolerant microbial communities in this area. Therefore, this study collected soils at distances of 15, 30, and 45 m from the Salt Lake and used non-saline soil (60 m) as a control to explore the microbial composition and salt tolerance mechanisms using metagenomics technology. The results showed that the dominant species and abundance of salt-tolerant microorganisms changed gradually with distance from Salt Lake. The salt-tolerant microorganisms can increase the expression of the Na+/H+ antiporter by upregulating the Na+/H+ antiporter subunit mnhA-G to respond to salt stress, simultaneously upregulating the genes in the betaine/proline transport system to promote the conversion of choline into betaine, while also upregulating the trehalose/maltose transport system encode genes to promote the synthesis of trehalose to resist a high salt environment.

Keywords