Molecules (Sep 2022)

Production of Salvianic Acid A from <span style="font-variant: small-caps">l</span>-DOPA via Biocatalytic Cascade Reactions

  • Ke Shun Hu,
  • Chong Le Chen,
  • Huan Ru Ding,
  • Tian Yu Wang,
  • Qin Zhu,
  • Yi Chen Zhou,
  • Jia Min Chen,
  • Jia Qi Mei,
  • Sheng Hu,
  • Jun Huang,
  • Wei Rui Zhao,
  • Le He Mei

DOI
https://doi.org/10.3390/molecules27186088
Journal volume & issue
Vol. 27, no. 18
p. 6088

Abstract

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Salvianic acid A (SAA), as the main bioactive component of the traditional Chinese herb Salvia miltiorrhiza, has important application value in the treatment of cardiovascular diseases. In this study, a two-step bioprocess for the preparation of SAA from l-DOPA was developed. In the first step, l-DOPA was transformed to 3,4-dihydroxyphenylalanine (DHPPA) using engineered Escherichia coli cells expressing membrane-bound L-amino acid deaminase from Proteus vulgaris. After that, the unpurified DHPPA was directly converted into SAA by permeabilized recombinant E. coli cells co-expressing d-lactate dehydrogenase from Pediococcus acidilactici and formate dehydrogenase from Mycobacterium vaccae N10. Under optimized conditions, 48.3 mM of SAA could be prepared from 50 mM of l-DOPA, with a yield of 96.6%. Therefore, the bioprocess developed here was not only environmentally friendly, but also exhibited excellent production efficiency and, thus, is promising for industrial SAA production.

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