The Antiproliferative Activity of <i>Adiantum pedatum</i> Extract and/or Piceatannol in Phenylhydrazine-Induced Colon Cancer in Male Albino Rats: The miR-145 Expression of the <i>PI-3K</i>/<i>Akt</i>/<i>p53</i> and <i>Oct4</i>/<i>Sox2</i>/<i>Nanog</i> Pathways
Tarek Khamis,
Abd Al-Aziz Abas Diab,
Mansour H. Zahra,
Samih Ebrahim El-Dahmy,
Basant Ahmed Abd Al-Hameed,
Adel Abdelkhalek,
Mahmoud A. Said,
Hussein Abdellatif,
Liana Mihaela Fericean,
Ioan Banatean-Dunea,
Ahmed Hamed Arisha,
Mai S. Attia
Affiliations
Tarek Khamis
Department of Pharmacology and Laboratory of Biotechnology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt
Abd Al-Aziz Abas Diab
Zoology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt
Mansour H. Zahra
Zoology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt
Samih Ebrahim El-Dahmy
Department of Pharmacology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt
Basant Ahmed Abd Al-Hameed
Zoology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt
Adel Abdelkhalek
Faculty of Veterinary Medicine, Badr University in Cairo, Badr City 11829, Egypt
Mahmoud A. Said
Zagazig University Hospital, Zagazig University, Zagazig 44511, Egypt
Hussein Abdellatif
Department of Human and Clinical Anatomy, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat 123, Oman
Liana Mihaela Fericean
Department of Biology, Faculty of Agriculture, University of Life Sciences, King Mihai I” from Timisoara [ULST], Aradului St. 119, 300645 Timisoara, Romania
Ioan Banatean-Dunea
Department of Biology, Faculty of Agriculture, University of Life Sciences, King Mihai I” from Timisoara [ULST], Aradului St. 119, 300645 Timisoara, Romania
Ahmed Hamed Arisha
Department of Animal Physiology and Biochemistry, Faculty of Veterinary Medicine, Badr University in Cairo, Badr City 11829, Egypt
Mai S. Attia
Zoology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt
Colon cancer is one of the most common types of cancer worldwide, and its incidence is increasing. Despite advances in medical science, the treatment of colon cancer still poses a significant challenge. This study aimed to investigate the potential protective effects of Adiantum pedatum (AP) extract and/or piceatannol on colon cancer induced via phenylhydrazine (PHZ) in terms of the antioxidant and apoptotic pathways and histopathologic changes in the colons of male albino rats. The rats were randomly divided into eight groups: control, AP extract, piceatannol (P), PHZ, PHZ and AP treatments, PHZ and P treatments, PHZ and both AP and P, and PHZ and prophylaxis with both AP and P. The results demonstrated that PHZ induced oxidative damage, apoptosis, and histopathological changes compared to the control group. However, the administration of AP or P or AP + P as therapy or prophylaxis significantly ameliorated these changes and upregulated the colonic mir-145 and mRNA expression of P53 and PDCD-4 while downregulating the colonic mRNA expression of PI3K, AKT, c-Myc, CK-20, SOX-2, OCT-4, and NanoG compared to the PHZ group. These findings suggest that the candidate drugs may exert their anti-cancer effects through multiple mechanisms, including antioxidant and apoptotic activities.
