Identification of Protease Producing Halophilic Bacteria from Bledug KuwuMud Volcano

Abstract

The objective of this research was to isolate and identify the halophilic bacteria from Bledug Kuwu-mud volacano having an ability to produce proteolytic enzyme. From this work, 6 bacterial isolates were obtained from mud and water samples using artificial sea water media after incubation at room temperature. Three out of the 6 isolates (BKL-3, BKL-5, and BKA-1) were selected for further analysis. BKL-3, BKL-5 and BKA-1 exhibited an ability to grow at salt concentration greater than 10%. BKL-3 could grow on media supplemented with 15% of salt, meanwhile BKL-5 and BKA-1 could grow at 20% of salt, respectively. Furthermore, those isolates also exhibited proteolytic activity when they were grown on casein media. The phylogenetic analysis based on the 16SrRNA gene sequences showed that the BKL-3 belong to the group of Bacillaceae, whilst BKL-5 and BKA-1 isolates were relatively distance from the group of Halomonadaceae. Therefore, BKL-5 and BKA-1 could be considered as the allegedly new species that were separated from Halomonadaceae

Keywords

• halophilic
• halotolerant
• protease
• 16SrRNA gene
• Bledug Kuwu-mud volcano
• phylogenetic tree