Animals (Jan 2023)

Effect of Detergents Based on Sodium Dodecyl Sulfate on Functional Metrics of Frozen–Thawed Collared Peccary (<i>Pecari tajacu</i>) Semen

  • Samara S. J. Moreira,
  • Andréia M. Silva,
  • Ana G. Pereira,
  • Romário P. Santos,
  • Maiko R. T. Dantas,
  • João B. F. Souza-Júnior,
  • Paola P. N. Snoeck,
  • Alexandre R. Silva

DOI
https://doi.org/10.3390/ani13030451
Journal volume & issue
Vol. 13, no. 3
p. 451

Abstract

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We evaluated the effects of detergents based on sodium dodecyl sulfoxide (SDS) on the functional parameters of collared peccary frozen–thawed sperm. Semen aliquots from ten individuals were diluted in a Tris–egg yolk–glycerol extender alone or with 0.5% Equex STM® paste or SDS (at 0.1%, 0.3% or 0.5% (v/v) concentration). Samples were fast frozen in liquid nitrogen with a post-thaw evaluation of motility, membrane functionality and integrity, mitochondrial activity, sperm binding ability and thermal resistance. The treatments without SDS (41.8 ± 3.5%) and those containing Equex (41.8 ± 4.4%) or 0.1% SDS (41.2 ± 5.5%) provided greater sperm motility (p p > 0.05), but SDS 0.5% impaired the membrane functionality and mitochondrial activity after thawing (p p < 0.05). Our results showed that the addition of 0.1% SDS to the Tris–yolk–glycerol extender optimized the freeze–thaw recovery of peccary semen.

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