lncRNA ILF3-AS1 promotes proliferation and metastasis of colorectal cancer cells by recruiting histone methylase EZH2

Sen Hong; Shiquan Li; Miaomiao Bi; Haiyao Yu; Zhenkun Yan; Tao Liu; Helei Wang

Molecular Therapy: Nucleic Acids (Jun 2021)

lncRNA ILF3-AS1 promotes proliferation and metastasis of colorectal cancer cells by recruiting histone methylase EZH2

Sen Hong,
Shiquan Li,
Miaomiao Bi,
Haiyao Yu,
Zhenkun Yan,
Tao Liu,
Helei Wang

Affiliations

Sen Hong: Department of Colorectal and Anal Surgery, The First Hospital of Jilin University, Changchun 130021, Jilin, People’s Republic of China
Shiquan Li: Department of Colorectal and Anal Surgery, The First Hospital of Jilin University, Changchun 130021, Jilin, People’s Republic of China
Miaomiao Bi: Department of Ophthalmology, The China-Japan Union Hospital of Jilin University, Jilin University, Changchun 130022, Jilin, People’s Republic of China
Haiyao Yu: Chief Pharmacist, Changchun Food and Drug Inspection Center, Changchun, Jilin, People’s Republic of China
Zhenkun Yan: Endoscopy Center, The China-Japan Union Hospital of Jilin University, Changchun 130022, Jilin, People’s Republic of China
Tao Liu: Department of Colorectal and Anal Surgery, The First Hospital of Jilin University, Changchun 130021, Jilin, People’s Republic of China
Helei Wang: Department of Gastrointestinal Surgery, The First Hospital of Jilin University, Changchun 130021, Jilin, People’s Republic of China; Corresponding author: Helei Wang, Department of Gastrointestinal Surgery, The First Hospital of Jilin University, Changchun 130021, Jilin, People’s Republic of China.

Journal volume & issue: Vol. 24
pp. 1012 – 1023

Abstract

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The role of long non-coding RNA (lncRNA) has been displayed in colorectal cancer (CRC). Here, we aimed to discuss the role of lncRNA interleukin enhancer-binding factor 3-antisense RNA 1 (ILF3-AS1)/enhancer of zeste homolog 2 (EZH2)/cyclin-dependent kinase inhibitor 2 (CDKN2A)/histone 3 (H3) lysine 27 trimethylation (H3K27me3) in cell proliferation and metastasis of CRC. ILF3-AS1, EZH2, and CDKN2A levels in CRC tissues and cells were detected. The relationship between ILF3-AS1/EZH2 expression and the clinicopathological features of CRC was analyzed. High/low expression of ILF3-AS1/EZH2 plasmids were composed to explore the function of ILF3-AS1/EZH2 in invasion, migration, proliferation, colony formation, and apoptosis of CRC cells. The growth status of nude mice was observed to verify the in vitro results from in vivo experiment. ILF3-AS1 and EZH2 increased, whereas CDKN2A reduced in CRC tissues and cells. ILF3-AS1 and EZH2 expression was linked to Dukes stage, distant metastasis, vascular invasion, and lymph node metastasis of CRC patients. Depleted ILF3-AS1 or reduced EZH2 suppressed proliferation, migration, colony-formation, and invasion ability, as well as facilitated apoptosis of CRC cells and attenuated the tumor growth in CRC mice. ILF3-AS1 accelerates the proliferation and metastasis of CRC cells by recruiting histone methylase EZH2 to induce trimethylation of H3K27 and downregulate CDKN2A.

Published in Molecular Therapy: Nucleic Acids

ISSN: 2162-2531 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://www.cell.com/molecular-therapy-family/nucleic-acids/latest-content

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