Profiling of Urinary Glucuronidated Bile Acids across Age Groups
Yamato Muto,
Mitsuyoshi Suzuki,
Genta Kakiyama,
Takahiro Sasaki,
Tsuyoshi Murai,
Hajime Takei,
Hiroshi Nittono
Affiliations
Yamato Muto
Department of Pediatrics, Faculty of Medicine, Juntendo University, Tokyo 113-8421, Japan
Mitsuyoshi Suzuki
Department of Pediatrics, Faculty of Medicine, Juntendo University, Tokyo 113-8421, Japan
Genta Kakiyama
Department of Internal Medicine, School of Medicine, Virginia Commonwealth University, 1101 E. Marshall St. Sanger Hall Suite 1-030, Richmond, VA 23298, USA
Takahiro Sasaki
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, 1757 Kanazawa, Tohbetsu-cho, Ishikari, Hokkaido 061-0293, Japan
Tsuyoshi Murai
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, 1757 Kanazawa, Tohbetsu-cho, Ishikari, Hokkaido 061-0293, Japan
Hajime Takei
Junshin Clinic Bile Acid Institute, 2-1-22 Haramachi, Meguro-ku, Tokyo 152-0011, Japan
Hiroshi Nittono
Junshin Clinic Bile Acid Institute, 2-1-22 Haramachi, Meguro-ku, Tokyo 152-0011, Japan
We investigated the age-dependent changes in urinary excretion of glucuronidated bile acids at the C-3 position. Bile acid 3-glucuronides accounted for 0.5% of urinary bile acids in neonates, and the proportion of bile acid 3-glucuronides plateaued at 1–3 years of age. The 3-glucuronides of secondary bile acids were first secreted at 3 months of age, the same time as the establishment of the gut bacterial flora in infants. A considerable portion of bile acid 3-glucuronides were present as non-amidated forms. Our results indicate dynamic hepatic enzyme activity in which the levels of uridine 5′-diphospho-glucuronosyltransferases (UGTs) differ by age group, with higher glucuronidation activity of UGTs towards nonamidated bile acids than amidated bile acids.
