Cells (Jul 2022)
Association of Clonal Hematopoiesis of Indeterminate Potential with Inflammatory Gene Expression in Patients with COPD
- Stefan Kuhnert,
- Siavash Mansouri,
- Michael A. Rieger,
- Rajkumar Savai,
- Edibe Avci,
- Gabriela Díaz-Piña,
- Manju Padmasekar,
- Mario Looso,
- Stefan Hadzic,
- Till Acker,
- Stephan Klatt,
- Jochen Wilhelm,
- Ingrid Fleming,
- Natascha Sommer,
- Norbert Weissmann,
- Claus Vogelmeier,
- Robert Bals,
- Andreas Zeiher,
- Stefanie Dimmeler,
- Werner Seeger,
- Soni S. Pullamsetti
Affiliations
- Stefan Kuhnert
- University of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Justus Liebig University, 35392 Giessen, Germany
- Siavash Mansouri
- Max Planck Institute for Heart and Lung Research, DZL, CPI, 61231 Bad Nauheim, Germany
- Michael A. Rieger
- Department of Medicine, Hematology/Oncology, University Hospital Frankfurt, CPI, Goethe University, 60596 Frankfurt am Main, Germany
- Rajkumar Savai
- University of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Justus Liebig University, 35392 Giessen, Germany
- Edibe Avci
- Max Planck Institute for Heart and Lung Research, DZL, CPI, 61231 Bad Nauheim, Germany
- Gabriela Díaz-Piña
- Max Planck Institute for Heart and Lung Research, DZL, CPI, 61231 Bad Nauheim, Germany
- Manju Padmasekar
- Max Planck Institute for Heart and Lung Research, DZL, CPI, 61231 Bad Nauheim, Germany
- Mario Looso
- Max Planck Institute for Heart and Lung Research, DZL, CPI, 61231 Bad Nauheim, Germany
- Stefan Hadzic
- University of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Justus Liebig University, 35392 Giessen, Germany
- Till Acker
- Institute for Neuropathology, CPI, Justus Liebig University, 35392 Giessen, Germany
- Stephan Klatt
- Institute of Vascular Signalling, Department of Molecular Medicine, CPI, Goethe University, 60596 Frankfurt am Main, Germany
- Jochen Wilhelm
- University of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Justus Liebig University, 35392 Giessen, Germany
- Ingrid Fleming
- Institute of Vascular Signalling, Department of Molecular Medicine, CPI, Goethe University, 60596 Frankfurt am Main, Germany
- Natascha Sommer
- University of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Justus Liebig University, 35392 Giessen, Germany
- Norbert Weissmann
- University of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Justus Liebig University, 35392 Giessen, Germany
- Claus Vogelmeier
- Department of Medicine, Pulmonary and Critical Care Medicine, Philipps University of Marburg, DZL, 35043 Marburg, Germany
- Robert Bals
- Department of Internal Medicine V-Pulmonology, Allergology and Critical Care Medicine, Saarland University, 66421 Homburg, Germany
- Andreas Zeiher
- Department of Medicine, Cardiology, CPI, Goethe University Hospital, 60596 Frankfurt am Main, Germany
- Stefanie Dimmeler
- Institute for Cardiovascular Regeneration, CPI, Goethe University, 60596 Frankfurt am Main, Germany
- Werner Seeger
- University of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Justus Liebig University, 35392 Giessen, Germany
- Soni S. Pullamsetti
- University of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Justus Liebig University, 35392 Giessen, Germany
- DOI
- https://doi.org/10.3390/cells11132121
- Journal volume & issue
-
Vol. 11,
no. 13
p. 2121
Abstract
Chronic obstructive pulmonary disease (COPD) is a disease with an inflammatory phenotype with increasing prevalence in the elderly. Expanded population of mutant blood cells carrying somatic mutations is termed clonal hematopoiesis of indeterminate potential (CHIP). The association between CHIP and COPD and its relevant effects on DNA methylation in aging are mainly unknown. Analyzing the deep-targeted amplicon sequencing from 125 COPD patients, we found enhanced incidence of CHIP mutations (~20%) with a predominance of DNMT3A CHIP-mediated hypomethylation of Phospholipase D Family Member 5 (PLD5), which in turn is positively correlated with increased levels of glycerol phosphocholine, pro-inflammatory cytokines, and deteriorating lung function.
Keywords
