Highly Accurate and Fast Electrochemical Detection of Scrub Typhus DNA via a Nanoflower NiFe-Based Biosensor
Fengzhen Li,
Delun Chen,
Wang He,
Juan Peng,
Yang Cao,
Jinchun Tu,
Xiaohong Wang
Affiliations
Fengzhen Li
Key Laboratory of Advanced Materials of Tropical Island Resources, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China
Delun Chen
Key Laboratory of Advanced Materials of Tropical Island Resources, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China
Wang He
Key Laboratory of Advanced Materials of Tropical Island Resources, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China
Juan Peng
State Key Lab High Efficiency Utilizat Coal & Gre, Natl Demonstrat Ctr Expt Chem Educ, Coll Chem & Chem Engn, Ningxia University, Yinchuan 750021, China
Yang Cao
Key Laboratory of Advanced Materials of Tropical Island Resources, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China
Jinchun Tu
Key Laboratory of Advanced Materials of Tropical Island Resources, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China
Xiaohong Wang
Key Laboratory of Advanced Materials of Tropical Island Resources, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China
Owing to the lack of specific diagnostic methods, Scrub typhus can sometimes be difficult to diagnose in the Asia-Pacific region. Therefore, an efficient and rapid detection method urgently needs to be developed. Based on competitive single-stranded DNA over modified glassy carbon electrode (GCE), an electrochemical biosensor was established to detect the disease. The nano-flower NiFe layered double hydroxide (NiFe-LDH) modified GCE has a large specific surface area, which supported a large amount of gold nanoparticles, so that a wide linear detection range from 25 fM to 0.5 μM was obtained. The beacon DNA (B-DNA) with the same sequence as the Scrub typhus DNA (T-DNA), but labeled with methylene blue, was used to construct a competitive relationship. When T-DNA and B-DNA were present on the sensor simultaneously, they would hybridize with probe DNA in a strong competition, and the corresponding electrochemical response signal would be generated via testing. It contributed to reducing tedious experimental procedures and excessive response time, and achieved fast electrochemical detection of DNA. The strategy provides a worthy avenue and possesses promising applications in disease diagnosis.
