Colicins of Escherichia coli Lead to Resistance against the Diarrhea-Causing Pathogen Enterotoxigenic E. coli in Pigs

Leli Wang; Yuwei Wu; Juan Xu; Qiuyun Huang; Ying Zhao; Sheng Dong; Xuxiang Wang; Xiaoni Cao; Chuni Wang; Anqi Wu; Diao Zhou; Cang Chen; Huansheng Yang; Jianzhong Li; Papadimitriou Konstantinos; Qiang Tu; Gaihua Zhang; Jia Yin

doi:10.1128/spectrum.01396-22

Microbiology Spectrum (Oct 2022)

Colicins of Escherichia coli Lead to Resistance against the Diarrhea-Causing Pathogen Enterotoxigenic E. coli in Pigs

Leli Wang,
Yuwei Wu,
Juan Xu,
Qiuyun Huang,
Ying Zhao,
Sheng Dong,
Xuxiang Wang,
Xiaoni Cao,
Chuni Wang,
Anqi Wu,
Diao Zhou,
Cang Chen,
Huansheng Yang,
Jianzhong Li,
Papadimitriou Konstantinos,
Qiang Tu,
Gaihua Zhang,
Jia Yin

Affiliations

Leli Wang: National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, China
Yuwei Wu: National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, China
Juan Xu: National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, China
Qiuyun Huang: National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, China
Ying Zhao: National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, China
Sheng Dong: National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, China
Xuxiang Wang: National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, China
Xiaoni Cao: National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, China
Chuni Wang: National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, China
Anqi Wu: National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, China
Diao Zhou: National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, China
Cang Chen: National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, China
Huansheng Yang: National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, China
Jianzhong Li: National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, China
Papadimitriou Konstantinos: Laboratory of Food Quality Control and Hygiene, Department of Food Science and Human Nutrition, Agricultural University of Athens, Athens, Greece
Qiang Tu: CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China
Gaihua Zhang: National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, China
Jia Yin: National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, China

DOI: https://doi.org/10.1128/spectrum.01396-22
Journal volume & issue: Vol. 10, no. 5

Abstract

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ABSTRACT Gut microbes can affect host adaptation to various environment conditions. Escherichia coli is a common gut species, including pathogenic strains and nonpathogenic strains. This study was conducted to investigate the effects of different E. coli strains in the gut on the health of pigs. In this study, the complete genomes of two E. coli strains isolated from pigs were sequenced. The whole genomes of Y18J and the enterotoxigenic E. coli strain W25K were compared to determine their roles in pig adaptation to disease. Y18J was isolated from feces of healthy piglets and showed strong antimicrobial activity against W25K in vitro. Gene knockout experiments and complementation analysis followed by modeling the microbe-microbe interactions demonstrated that the antagonistic mechanism of Y18J against W25K relied on the bacteriocins colicin B and colicin M. Compared to W25K, Y18J is devoid of exotoxin-coding genes and has more secondary-metabolite-biosynthetic gene clusters. W25K carries more genes involved in genome replication, in accordance with a shorter cell cycle observed during a growth experiment. The analysis of gut metagenomes in different pig breeds showed that colicins B and M were enriched in Laiwu pigs, a Chinese local breed, but were scarce in boars and Duroc pigs. IMPORTANCE This study revealed the heterogeneity of E. coli strains from pigs, including two strains studied by both in silico and wet experiments in detail and 14 strains studied by bioinformatics analysis. E. coli Y18J may improve the adaptability of pigs toward disease resistance through the production of colicins B and M. Our findings could shed light on the pathogenic and harmless roles of E. coli in modern animal husbandry, leading to a better understanding of intestinal-microbe–pathogen interactions in the course of evolution.

Published in Microbiology Spectrum

ISSN: 2165-0497 (Online)
Publisher: American Society for Microbiology
Country of publisher: United States
LCC subjects: Science: Microbiology
Website: https://journals.asm.org/journal/spectrum

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