Transcriptome Analysis and Identification of Chemosensory Genes in <i>Baryscapus dioryctriae</i> (Hymenoptera: Eulophidae)
Xiaoyan Zhu,
Qiling Yu,
Xingyu Gan,
Liwen Song,
Kaipeng Zhang,
Tongtong Zuo,
Junjie Zhang,
Ying Hu,
Qi Chen,
Bingzhong Ren
Affiliations
Xiaoyan Zhu
Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, School of Life Sciences, Northeast Normal University, Changchun 130024, China
Qiling Yu
Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, School of Life Sciences, Northeast Normal University, Changchun 130024, China
Xingyu Gan
Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, School of Life Sciences, Northeast Normal University, Changchun 130024, China
Liwen Song
Research Institute of Forest Protection, Jilin Provincial Academy of Forestry Sciences, Changchun 130033, China
Kaipeng Zhang
Research Institute of Forest Protection, Jilin Provincial Academy of Forestry Sciences, Changchun 130033, China
Tongtong Zuo
Research Institute of Forest Protection, Jilin Provincial Academy of Forestry Sciences, Changchun 130033, China
Junjie Zhang
Engineering Research Center of Natural Enemies, Institute of Biological Control, Jilin Agricultural University, Changchun 130118, China
Ying Hu
Engineering Research Center of Natural Enemies, Institute of Biological Control, Jilin Agricultural University, Changchun 130118, China
Qi Chen
Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, School of Life Sciences, Northeast Normal University, Changchun 130024, China
Bingzhong Ren
Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, School of Life Sciences, Northeast Normal University, Changchun 130024, China
Baryscapus dioryctriae is a pupal endoparasitoid of many Pyralidae pests and has been used as a biocontrol agent against insect pests that heavily damage the cone and seed of the Korean pine. The olfactory system of wasps plays an essential role in sensing the chemical signals during their foraging, mating, host location, etc., and the chemosensory genes are involved in detecting and transducing these signals. Many chemosensory genes have been identified from the antennae of Hymenoptera; however, there are few reports on the chemosensory genes of Eulophidae wasps. In this study, the transcriptome databases based on ten different tissues of B. dioryctriae were first constructed, and 274 putative chemosensory genes, consisting of 27 OBPs, 9 CSPs, 3 NPC2s, 155 ORs, 49 GRs, 23 IRs and 8 SNMPs genes, were identified based on the transcriptomes and manual annotation. Phylogenetic trees of the chemosensory genes were constructed to investigate the orthologs between B. dioryctriae and other insect species. Additionally, twenty-eight chemosensory genes showed female antennae- and ovipositor-biased expression, which was validated by RT-qPCR. These findings not only built a molecular basis for further research on the processes of chemosensory perception in B. dioryctriae, but also enriched the identification of chemosensory genes from various tissues of Eulophidae wasps.
