mSystems (Jun 2021)

High-Content Imaging to Phenotype Antimicrobial Effects on Individual Bacteria at Scale

  • Sushmita Sridhar,
  • Sally Forrest,
  • Ben Warne,
  • Mailis Maes,
  • Stephen Baker,
  • Gordon Dougan,
  • Josefin Bartholdson Scott

DOI
https://doi.org/10.1128/mSystems.00028-21
Journal volume & issue
Vol. 6, no. 3

Abstract

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High-content imaging (HCI) is a microscopy technique that permits the screening of multiple cells simultaneously in high resolution to detect subtle morphological and phenotypic variation. The power of this methodology is that it can generate large data sets comprised of multiple parameters taken from individual cells subjected to a range of different conditions.