Dataset of Partial Analytical Validation of the 1,2-O-Dilauryl-Rac-Glycero-3-Glutaric Acid-(6′-Methylresorufin) Ester (DGGR) Lipase Assay in Equine Plasma

Abstract

Laboratory assays require analytical validation to prove they are providing accurate results. This dataset describes the partial analytical validation of lipase activity, measured with the 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6′-methylresorufin) ester (DGGR) lipase assay in equine plasma. Samples with low (approx. 12 U/L), moderately increased (approx. 79 U/L), and markedly increased lipase activity (approx. 298 U/L) were chosen. Linearity was assessed in samples of ascending dilution prepared by mixing samples with low and high lipase activity in different proportions. Repeatability or intra-assay replication was evaluated by measuring each level in 25 replicates within the same run. Reproducibility or inter-assay replication was calculated by measuring each level in five replicates on five consecutive days. The assay was linear in the range of 12–298 U/L (R2 = 0.9998) with a <2.3% deviation from the calculated value at any point. Within-run coefficients of variation were 4.43%, 0.69%, and 1.00% for the low, medium, and high samples, respectively. Between-run coefficients of variation were 3.57%, 1.42%, and 1.16%, respectively. To our knowledge, these are the first published data on the analytical validation of the DGGR lipase assay in horses, which may be of interest to veterinary clinical pathologists and equine clinicians measuring DGGR lipase in equine blood for diagnostic and research purposes.

Keywords

• horse
• pancreas
• enzyme
• linearity
• repeatability
• reproducibility