Expression Profiles of Genes Encoded by the Supernumerary Chromosome Controlling AM-Toxin Biosynthesis and Pathogenicity in the Apple Pathotype of Alternaria alternata

Abstract

The apple pathotype of Alternaria alternata produces host-specific AM-toxin and causes Alternaria blotch of apple. Previously, we cloned two genes, AMT1 and AMT2, required for AM-toxin biosynthesis and found that these genes are encoded by small, supernumerary chromosomes of 10-fold) in AM-toxin–producing cultures. Two of the upregulated genes were newly identified to be involved in AM-toxin biosynthesis by the gene disruption experiments and were named AMT3 and AMT4. Thus, the genes upregulated in AM-toxin–producing cultures contain ideal candidates for novel AM-toxin biosynthetic genes.

Keywords

• host-specific toxin