Frontiers in Pediatrics (May 2017)

Umbilical Cord Blood NOS1 as a Potential Biomarker of Neonatal Encephalopathy

  • Jun Lei,
  • Cristina Paules,
  • Elisabeth Nigrini,
  • Jason M. Rosenzweig,
  • Rudhab Bahabry,
  • Azadeh Farzin,
  • Samuel Yang,
  • Frances J. Northington,
  • Frances J. Northington,
  • Frances J. Northington,
  • Daniel Oros,
  • Stephanie McKenney,
  • Michael V. Johnston,
  • Michael V. Johnston,
  • Michael V. Johnston,
  • Ernest M. Graham,
  • Ernest M. Graham,
  • Irina Burd,
  • Irina Burd,
  • Irina Burd,
  • Irina Burd

DOI
https://doi.org/10.3389/fped.2017.00112
Journal volume & issue
Vol. 5

Abstract

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BackgroundThere are no definitive markers to aid in diagnosis of neonatal encephalopathy (NE). The purpose of our study was (1) to identify and evaluate the utility of neuronal nitric oxide synthase (NOS1) in umbilical cord blood as a NE biomarker and (2) to identify the source of NOS1 in umbilical cord blood.MethodsThis was a nested case–control study of neonates >35 weeks of gestation. ELISA for NOS1 in umbilical cord blood was performed. Sources of NOS1 in umbilical cord were investigated by immunohistochemistry, western blot, ELISA, and quantitative PCR. Furthermore, umbilical cords of full-term neonates were subjected to 1% hypoxia ex vivo.ResultsNOS1 was present in umbilical cord blood and increased in NE cases compared with controls. NOS1 was expressed in endothelial cells of the umbilical cord vein, but not in artery or blood cells. In ex vivo experiments, hypoxia was associated with increased levels of NOS1 in venous endothelial cells of the umbilical cord as well as in ex vivo culture medium.ConclusionThis is the first study to investigate an early marker of NE. NOS1 is elevated with hypoxia, and further studies are needed to investigate it as a valuable tool for early diagnosis of neonatal brain injury.

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