Recent advances in infectious disease research using cryo-electron tomography

Daniel Asarnow; Vada A. Becker; Daija Bobe; Charlie Dubbledam; Jake D. Johnston; Jake D. Johnston; Mykhailo Kopylov; Nathalie R. Lavoie; Qiuye Li; Jacob M. Mattingly; Joshua H. Mendez; Mohammadreza Paraan; Jack Turner; Viraj Upadhye; Richard M. Walsh; Meghna Gupta; Edward T. Eng

doi:10.3389/fmolb.2023.1296941

Frontiers in Molecular Biosciences (Jan 2024)

Recent advances in infectious disease research using cryo-electron tomography

Daniel Asarnow,
Vada A. Becker,
Daija Bobe,
Charlie Dubbledam,
Jake D. Johnston,
Jake D. Johnston,
Mykhailo Kopylov,
Nathalie R. Lavoie,
Qiuye Li,
Jacob M. Mattingly,
Joshua H. Mendez,
Mohammadreza Paraan,
Jack Turner,
Viraj Upadhye,
Richard M. Walsh,
Meghna Gupta,
Edward T. Eng

Affiliations

Daniel Asarnow: Department of Biochemistry, University of Washington, Seattle, WA, United States
Vada A. Becker: Department of Medicinal Chemistry, University of Washington, Seattle, WA, United States
Daija Bobe: Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States
Charlie Dubbledam: Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States
Jake D. Johnston: Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States
Jake D. Johnston: Department of Physiology and Cellular Biophysics, Columbia University, New York, NY, United States
Mykhailo Kopylov: Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States
Nathalie R. Lavoie: Department of Molecular Biology and Microbiology, School of Medicine, Tufts University, Boston, MA, United States
Qiuye Li: Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, OH, United States
Jacob M. Mattingly: Department of Chemistry, College of Arts and Sciences, Emory University, Atlanta, GA, United States
Joshua H. Mendez: Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States
Mohammadreza Paraan: Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States
Jack Turner: European Bioinformatics Institute (EMBL-EBI), Cambridge, United Kingdom
Viraj Upadhye: Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
Richard M. Walsh: 0Harvard Cryo-Electron Microscopy Center for Structural Biology and Harvard Medical School, Boston, MA, United States
Meghna Gupta: 1Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA, United States
Edward T. Eng: Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States

DOI: https://doi.org/10.3389/fmolb.2023.1296941
Journal volume & issue: Vol. 10

Abstract

Read online

With the increasing spread of infectious diseases worldwide, there is an urgent need for novel strategies to combat them. Cryogenic sample electron microscopy (cryo-EM) techniques, particularly electron tomography (cryo-ET), have revolutionized the field of infectious disease research by enabling multiscale observation of biological structures in a near-native state. This review highlights the recent advances in infectious disease research using cryo-ET and discusses the potential of this structural biology technique to help discover mechanisms of infection in native environments and guiding in the right direction for future drug discovery.

Published in Frontiers in Molecular Biosciences

ISSN: 2296-889X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.frontiersin.org/journals/molecular-biosciences

About the journal

Abstract

Keywords