Journal of Pharmacological Sciences (Jan 2011)

Effect of Cigarette Smoke Components on Vascular Smooth Muscle Cell Migration Toward Platelet-Derived Growth Factor BB

  • Shinji Yoshiyama,
  • Takahiro Horinouchi,
  • Soichi Miwa,
  • Hong Hui Wang,
  • Kazuhiro Kohama,
  • Akio Nakamura

DOI
https://doi.org/10.1254/jphs.10283sc
Journal volume & issue
Vol. 115, no. 4
pp. 532 – 535

Abstract

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Cigarette smoking is one of the factors causing accumulation of vascular smooth muscle cells (VSMCs) in atherosclerotic plaques. Changes in cell migration toward platelet-derived growth factor BB were investigated using a Boyden chamber after 48-h preincubation of GBaSM-4 VSMCs with nicotine or nicotine-free cigarette smoke extract (CSE). A nicotine concentration of 0.1 μM maximally promoted cell migration; 0.1% CSE also promoted cell migration, while high CSE concentrations damaged GBaSM-4 cells. Fetal bovine serum (FBS) long-depletion induced decrease in migration of GBaSM-4 cells. Our results suggest that nicotine and some CSE components can induce GBaSM-4 cell migration. Keywords:: smooth muscle, nicotine, cigarette smoke extract (CSE)