Communications Biology (Oct 2021)

Subdiffraction-resolution fluorescence imaging of immunological synapse formation between NK cells and A. fumigatus by expansion microscopy

  • Nora Trinks,
  • Sebastian Reinhard,
  • Matthias Drobny,
  • Linda Heilig,
  • Jürgen Löffler,
  • Markus Sauer,
  • Ulrich Terpitz

DOI
https://doi.org/10.1038/s42003-021-02669-y
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 12

Abstract

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Trinks et al developed a protocol that enables super-resolved visualisation of the interaction between natural killer cells and Aspergillus fumigatus hyphae by expansion microscopy. They describe in detail steps of the formation of the immunological synapse including cytoskeleton re-arrangement, granule convergence and translocation of LAMP1, demonstrating the utility of the method.