Protocol for studying macrophage lipid crosstalk with murine tumor cells

Abstract

Summary: Lipid accumulation has recently emerged as a key feature underlying the pro-tumorigenic role of macrophages. Here, we present a workflow to study macrophage lipid crosstalk with tumor cells. We describe steps for the identification, purification, and multi-omics characterization of lipid-laden macrophages (LLMs) from murine tumors and outline protocols to assess the functional significance of LLMs in cancer malignancy. This approach has the potential to uncover the source of lipids that drives LLM formation and its pro-tumorigenic potential in multiple cancer types.For complete details on the use and execution of this protocol, please refer to Kloosterman, Erbani, et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• bioinformatics
• sequence analysis
• cell biology
• single cell
• flow cytometry
• cancer