Phylogenetic tree analysis study of Lumpy skin disease virus based envelope protein P32 gene in Al-Qadisiyah province, Iraq

Abstract

In this study, fifty samples from nodules of skin tissue collected from cattle were clinically supposed to be infected with lumpy skin disease (LSD) from deferent area in Al-Qadisiyah province .These specimens were provided to PCR assay. The endpoint of conventional PCR assay was sent to sequence analysis. The results revealed that thirty-nine samples out of fifty were positive to lumpy skin disease virus at 752bp PCR product of p32 gene, the sequence analysis of five positive samples were done as a confirmative diagnosis to PCR assay. The sequences of these five samples can be found under the accession numbers (KR066462.1,KR066463.1,KR066464.1,KR066465.1 and KR066466.1) at NCBI-Gen Bank submission. In conclusion; the sequence analysis of five local isolate of lumpy skin disease virus was close related to NCBI-Blast reference lumpy skin disease virus isolate (KU720359.1) and Kurdistan isolates, whereas others "Turkish and Egyptian isolates" were different.