Construction of tissue-engineered nucleus pulposus by stimulation with periodic mechanical stress and BMP-2

Yang Liu; Gong-ming Gao; Kai-yuan Yang; Lu-ming Nong

iScience (Jun 2022)

Construction of tissue-engineered nucleus pulposus by stimulation with periodic mechanical stress and BMP-2

Yang Liu,
Gong-ming Gao,
Kai-yuan Yang,
Lu-ming Nong

Affiliations

Yang Liu: Department of Orthopedics, The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical University, No.29 Xinglong Lane, Tianning District, Changzhou, Jiangsu Province 213000, P.R. China; Dalian Medical University, No. 9 West Section of South Lvshun Road, Lvshunkou District, Dalian, Liaoning Province 116044, P.R. China
Gong-ming Gao: Department of Orthopedics, The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical University, No.29 Xinglong Lane, Tianning District, Changzhou, Jiangsu Province 213000, P.R. China
Kai-yuan Yang: Department of Orthopedics, The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical University, No.29 Xinglong Lane, Tianning District, Changzhou, Jiangsu Province 213000, P.R. China
Lu-ming Nong: Department of Orthopedics, The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical University, No.29 Xinglong Lane, Tianning District, Changzhou, Jiangsu Province 213000, P.R. China; Corresponding author

Journal volume & issue: Vol. 25, no. 6
p. 104405

Abstract

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Summary: Intervertebral disc (IVD) degeneration, which is common among elderly individuals, mainly manifests as low back pain and is caused by structural deterioration of the nucleus pulposus (NP) due to physiological mechanical stress. NP mesenchymal stem cells (NPMSCs) around the IVD endplate have multidirectional differentiation potential and can be used for tissue repair. To define favorable conditions for NPMSC proliferation and differentiation into chondroid cells for NP repair, the present study simulated periodic mechanical stress (PMS) of the NP under physiological conditions using MSC chondrogenic differentiation medium and recombinant human BMP-2 (rhBMP-2). rhBMP-2 effectively promoted NPMSC proliferation and differentiation. To clarify the mechanism of action of rhBMP-2, integrin alpha 1 (ITG A1) and BMP-2 were inhibited. PMS regulated the BMP-2/Smad1/RUNX2 pathway through ITG A1 and promoted NPMSC proliferation and differentiation. During tissue-engineered NP construction, PMS can effectively reduce osteogenic differentiation and promote extracellular matrix protein synthesis to enhance structural NP recovery.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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Abstract

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