Quantifying Plant Viruses: Evolution from Bioassay to Infectivity Dilution Curves along the Model of Tobamoviruses
Shaheen Nourinejhad Zarghani,
Mehran Monavari,
Amin Nourinejhad Zarghani,
Sahar Nouri,
Jens Ehlers,
Joachim Hamacher,
Martina Bandte,
Carmen Büttner
Affiliations
Shaheen Nourinejhad Zarghani
Division Phytomedicine, Faculty of Life Sciences, Albrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Humboldt-Universität in Berlin, Lentzeallee 55–57, 14197 Berlin, Germany
Mehran Monavari
Section eScience, Federal Institute for Materials Research and Testing, Unter den Eichen 87, 12205 Berlin, Germany
Amin Nourinejhad Zarghani
School of Mechanical Engineering, Hamburg University of Technology, Eissendorfer Str. 38, 21073 Hamburg, Germany
Sahar Nouri
Division Phytomedicine, Faculty of Life Sciences, Albrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Humboldt-Universität in Berlin, Lentzeallee 55–57, 14197 Berlin, Germany
Jens Ehlers
Division Phytomedicine, Faculty of Life Sciences, Albrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Humboldt-Universität in Berlin, Lentzeallee 55–57, 14197 Berlin, Germany
Joachim Hamacher
Institute of Crop Science and Resource Conservation (INRES)—Plant Pathology, Universität Bonn, Nussallee 9, 53115 Bonn, Germany
Martina Bandte
Division Phytomedicine, Faculty of Life Sciences, Albrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Humboldt-Universität in Berlin, Lentzeallee 55–57, 14197 Berlin, Germany
Carmen Büttner
Division Phytomedicine, Faculty of Life Sciences, Albrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Humboldt-Universität in Berlin, Lentzeallee 55–57, 14197 Berlin, Germany
This review describes the development of the bioassay as a means of quantifying plant viruses, with particular attention to tobamovirus. It delves into various models used to establish a correlation between virus particle concentration and the number of induced local lesions (the infectivity dilution curve), including the Poisson, Furumoto and Mickey, Kleczkowski, Growth curve, and modified Poisson models. The parameters of each model are described, and their application or performance in the context of the tobacco mosaic virus is explored. This overview highlights the enduring value of the infectivity dilution curve in tobamovirus quantification, providing valuable insights for researchers or practitioners of bioassays and theoreticians of modeling.
