MECHANISMS OF ANTI-PROLIFERATIVE EFFECT PRODUCED BY MESENCHYMAL STEM CELLS IN PATIENTS WITH MULTIPLE SCLEROSIS

Abstract

Abstract. We investigated the ability of autologous/allogeneic mesenchymal stem cells (MSC) from early passages, derived from bone marrow of patients with multiple sclerosis, to inhibit mitogen/myelin-induced proliferation of memory T cells. It was shown that MSC immunosuppressive potential of myelin-induced T-cell memory proliferation was significantly higher than an appropriate suppressive effect upon mitogenstimulated cells. These data provide an evidence for a possible pathogenetic role of MSCs in suppression of myelin-specific proliferation of effector lymphoid cells. Immunoregulatory mechanisms of mesenchymal stem cells have been determined. It has been shown that both soluble factors and cell-mediated interactions may be involved in immunosuppressive activity of MSCs. Moreover, soluble mediators of MSC immunoregulatory properties, e.c., prostaglandin E2 and indoleamine 2,3-dioxygenase, were not produced in constitutive manner, but they require a paracrine signal from T-lymphocytes. The data obtained may be used for development of an individual approach, in order to estimate immunomodulatory properties of MSCs and for further application of cell cultures in pathogenetic therapy of multiple sclerosis. (Med. Immunol., 2011, vol. 13, N 2-3, pp 237-246)

Keywords

• immune suppression
• mesenchymal stem cells
• multiple sclerosis
• prostaglandin e2
• indoleamine 2
• 3-dioxygenase