Polymorphism Exploration of Growth Family (GH, GHRH and PIT-1) Genes Polymorphisms of Local Swamp Buffalo for Productivity Improvement in North Tapanuli Regency, North Sumatra

Abstract

Genetic improvement of livestock productivity can be done through molecular selection on the genes controlling growth traits. Genetic polymorphism of the growth family (GH, GHRH, and PIT1) genes were studied in local swamp buffalo (106 hds.) from a government buffalo breeding station (46 heads) and smallholders (60 heads) in North Tapanuli District, North Sumatra Province. Genotype variants of the three genes were identified by PCR-RFLP method using restriction enzymes of MspI (GH gene), HaeIII (GHRH gene) and HinfI (PIT -1 gene). Genotyping on individual GH_g.1547T>C, GHRH_g.4666G>C, and PIT -1_g.1256G >A loci resulted only one type genotype, respectively TT, CC, an d AA, with one type of allele, respectively T, C, and A. Heterozygosity observation (Ho) and expectation (He) values values and the PIC value for each locus was 0.00. It could be suggested to increase genotype frequenciest of the three growth genes that are positively associated with the growth traits and economic traits of the buffalo.

Keywords

• growth family genes
• pcr-rflp
• swamp buffalo.