Nature Communications (Mar 2021)
Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics
- Lars Velten,
- Benjamin A. Story,
- Pablo Hernández-Malmierca,
- Simon Raffel,
- Daniel R. Leonce,
- Jennifer Milbank,
- Malte Paulsen,
- Aykut Demir,
- Chelsea Szu-Tu,
- Robert Frömel,
- Christoph Lutz,
- Daniel Nowak,
- Johann-Christoph Jann,
- Caroline Pabst,
- Tobias Boch,
- Wolf-Karsten Hofmann,
- Carsten Müller-Tidow,
- Andreas Trumpp,
- Simon Haas,
- Lars M. Steinmetz
Affiliations
- Lars Velten
- Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
- Benjamin A. Story
- European Molecular Biology Laboratory (EMBL), Genome Biology Unit
- Pablo Hernández-Malmierca
- Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH)
- Simon Raffel
- Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH)
- Daniel R. Leonce
- European Molecular Biology Laboratory (EMBL), Genome Biology Unit
- Jennifer Milbank
- European Molecular Biology Laboratory (EMBL), Genome Biology Unit
- Malte Paulsen
- European Molecular Biology Laboratory (EMBL), Flow Cytometry Core Facility
- Aykut Demir
- Department of Internal Medicine V, Hematology, Oncology and Rheumatology, University of Heidelberg
- Chelsea Szu-Tu
- Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
- Robert Frömel
- Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
- Christoph Lutz
- Department of Internal Medicine V, Hematology, Oncology and Rheumatology, University of Heidelberg
- Daniel Nowak
- Department of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University
- Johann-Christoph Jann
- Department of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University
- Caroline Pabst
- Department of Internal Medicine V, Hematology, Oncology and Rheumatology, University of Heidelberg
- Tobias Boch
- Swiss Federal Institute of Technology (ETH) Zurich, Department of Biosystems Science and Engineering
- Wolf-Karsten Hofmann
- Department of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University
- Carsten Müller-Tidow
- Department of Internal Medicine V, Hematology, Oncology and Rheumatology, University of Heidelberg
- Andreas Trumpp
- Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH)
- Simon Haas
- Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH)
- Lars M. Steinmetz
- European Molecular Biology Laboratory (EMBL), Genome Biology Unit
- DOI
- https://doi.org/10.1038/s41467-021-21650-1
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 13
Abstract
Leukaemic stem cells drive acute myeloid leukaemia (AML) progression and relapse but they are incompletely characterized. Here, the authors combine single-cell transcriptomics and clonal tracking using nuclear and mitochondrial somatic variants to distinguish healthy, pre-leukaemic and leukaemic stem cells in AML.
