ANALYSIS OF CONTROL MEASUREMENTS OF «T-CELL REPLICATES» AND «T-CELL SUMMARY» IN INVESTIGATION OF THE IMMUNE STATUS OF HIV-INFECTED PATIENTS AND DETECTION DOUBLE NEGATIVE SUBPOPULATION OF T-LYMPHOCYTES (CD45+CD3+CD4–CD8–)

Abstract

Abstract. We analyzed efficiency of application for analytic control parameters, i.e., «T-cell replicates» and «T-cell summary» in a sample of 174 HIV-infected patients, independently of age or disease stage. The patients were divided in two groups. Group 1 consisted of patients with total CD45+CD3+CD4+ and CD45+CD3+CD8+ lymphocyte concentrations being similar to relative and absolute concentrations of CD45+CD3+ lymphocytes, or the difference between absolute measurements not exceeding «T-cell replicates» differences. Group 1 included 36.78% of the whole cohort. Group 2 consisted of patients with total CD45+CD3+CD4+ & CD45+CD3+CD8+ lymphocytes concentrations being lower than relative and absolute concentrations of CD45+CD3+ lymphocytes, and the differences in absolute values being higher than «T-cell replicates» differences (41.38% of total sample). Such difference between control sums may be connected with high concentrations of double-negative γδ-T-cells (CD45+CD3+CD4–CD8–) in the specimens under study. (Med. Immunol., 2008, vol. 10, N 4-5, pp 463-466).

Keywords

• flow cytometry
• t-lymphocytes
• hiv