Frontiers in Plant Science (Apr 2016)

Genome wide SSR high density genetic map construction from an interspecific cross of Gossypium hirsutum × Gossypium tomentosum

  • Muhammad Kashif Riaz eKhan,
  • Muhammad Kashif Riaz eKhan,
  • Haodong eChen,
  • Haodong eChen,
  • Zhongli eZhou,
  • Muhammad Kashif eIlyas,
  • Muhammad Kashif eIlyas,
  • Xingxing eWang,
  • Xiaoyan eCai,
  • Chunying eWang,
  • Fang eLiu,
  • Kunbo eWang

DOI
https://doi.org/10.3389/fpls.2016.00436
Journal volume & issue
Vol. 7

Abstract

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A high density genetic map was constructed using F2 population derived from an interspecific cross of G. hirsutum x G. tomentosum. The map consisted of 3,093 marker loci distributed across all the 26 chromosomes and covered 4,365.3 cM of cotton genome with an average inter-marker distance of 1.48 cM. The maximum length of chromosome was 218.38 cM and the minimum was 122.09 cM with an average length of 167.90 cM. A sub-genome covers more genetic distance (2,189.01 cM) with an average inter loci distance of 1.53 cM than D sub-genome which covers a length of 2,176.29 cM with an average distance of 1.43 cM. There were 716 distorted loci in the map accounting for 23.14% and most distorted loci were distributed on D sub-genome (25.06%), which were more than on A sub-genome (21.23%). In our map 49 segregation hotspots (SDR) were distributed across the genome with more on D sub-genome as compared to A genome. Two post-polyploidization reciprocal translocations of A2/A3 and A4/A5 were suggested by 7 pairs of duplicate loci. The map constructed through these studies is one of the three densest genetic maps in cotton however; this is the first dense genome wide SSR interspecific genetic map between G. hirsutum and G. tomentosum.

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