STRENGTHENING OF ORBITAL STUMP USING AUTOLOGOUS BLOOD MONONUCLEAR CELLS IN EXPERIMENT

Abstract

Purpose. To study the morphological characteristics of a course of inflammatory and reparative processes in the formation of the orbital stump with a bioengineered complex of nickel titanium and autologous blood mononuclear cells in experiment.Material and methods. A series of experiments was performed in 36 adult Wistar rats (weight: 200-250g). After an ether anesthesia, each animal underwent evisceroenucleation followed by insertion the implant directly into the scleral bag. In the main group of animals the culture of autologous blood mononuclear cells was injected into the structure of implant. The material sampling was carried out on 7, 14, 21 days from the start of the experiment.Results. The obtained results showed that the specific volume of connective tissue, starting from 7 days after surgery prevailed in the main group and in all periods of the experiment were significantly higher than those in the comparative group. A significantly higher quantity of neo-capillaries with a wide lumen was also detected in the main group, unlike the comparative group.Conclusions. Thus, the study showed that the mononuclear cells stimulate fibroblast proliferation and connection to the reparative processes, angiogenesis and pronounced collagen formation in the orbital cavity, which promotes a more robust strengthening and retain of the implant in the orbital cavity. Consequently, the proposed method is a new and effective option to promote orbital stump in less time and requires significantly lower material costs and can be recommended for clinical trials.

Keywords

• titanium nickelide
• orbital stump
• mononuclear cells
• evisceroenucleation