Investigation of the Penetration Ability of InP/ZnSe/ZnS 650 Quantum Dots into the Anterior Chamber of the Eye by Topical Application. Experimental Study

Abstract

In connection with the problem of formation of strains of pathogenic microflora resistant to antibacterial drugs, research on the use of nanoparticles, namely quantum dots, has been actively conducted recently. Quantum dots can be used as an anti-infective agent, a means for visualization of intraocular structures, drug delivery, as well as a means for electrical stimulation of the retina. Successful application of quantum dots as an anti-infective agent necessitates the study of their penetrating ability into the anterior chamber. The aim of the study was to experimentally investigate the penetration ability of InP/ZnSe/ZnS 650 QDs in the anterior chamber of the eye at topical application. The object of the study was InP/ZnSe/ZnS 650 quantum dots. The study was carried out on laboratory rabbits (#6), which were divided randomly in pairs into groups. In rabbits #1 and #2 the eyes remained intact, #3 and #4 — a bandage soft contact lens was placed on the cornea of the right eye, #5 and #6 the cornea of the right eye was de-epithelialized. All experimental rabbits during the day 6 times instillation of 10 % solution of InP/ZnSe/ZnS 650 quantum dots was performed, and at the end 0.2 ml of moisture was taken from the anterior chamber of the right eye. The left eyes in all individuals were the control group. Identification of quantum dots into the anterior chamber was performed using a highly sensitive spectrophotometer UV-3600 (Shimadzu). The experimental study of the ability of InP/ZnSe/ZnS 650 quantum dots at topical application to penetrate into the intraocular cavity by the claimed detection methods did not allow their detection in the anterior chamber moisture even in minimal concentration.

Keywords

• quantum dots
• cornea
• penetrating ability
• topical application