Aggregated carbon dots‐loaded macrophages treat sepsis by eliminating multidrug‐resistant bacteria and attenuating inflammation
Lihua Li,
Lingling Chen,
Yao Lu,
Binglin Li,
Rong Hu,
Ling Huang,
Tao Zhang,
Xiaoming Wei,
Zhongmin Yang,
Chuanbin Mao
Affiliations
Lihua Li
State Key Laboratory of Luminescent Materials and Devices Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques School of Materials Science and Engineering South China University of Technology Guangzhou Guangdong P. R. China
Lingling Chen
Guangdong Key Lab of Orthopedic Technology and Implant General Hospital of Southern Theater Command of PLA The First School of Clinical Medicine Southern Medical University Guangzhou Guangdong P. R. China
Yao Lu
Orthopedic Centre Clinical Research Centre Zhujiang Hospital Southern Medical University Guangzhou Guangdong P. R. China
Binglin Li
Guangdong Key Lab of Orthopedic Technology and Implant General Hospital of Southern Theater Command of PLA The First School of Clinical Medicine Southern Medical University Guangzhou Guangdong P. R. China
Rong Hu
State Key Laboratory of Luminescent Materials and Devices Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques School of Materials Science and Engineering South China University of Technology Guangzhou Guangdong P. R. China
Ling Huang
State Key Laboratory of Luminescent Materials and Devices Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques School of Materials Science and Engineering South China University of Technology Guangzhou Guangdong P. R. China
Tao Zhang
Guangdong Key Lab of Orthopedic Technology and Implant General Hospital of Southern Theater Command of PLA The First School of Clinical Medicine Southern Medical University Guangzhou Guangdong P. R. China
Xiaoming Wei
State Key Laboratory of Luminescent Materials and Devices Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques School of Materials Science and Engineering South China University of Technology Guangzhou Guangdong P. R. China
Zhongmin Yang
State Key Laboratory of Luminescent Materials and Devices Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques School of Materials Science and Engineering South China University of Technology Guangzhou Guangdong P. R. China
Chuanbin Mao
School of Materials Science and Engineering Zhejiang University Hangzhou Zhejiang P. R. China
Abstract Sepsis, caused by uncontrollable infection and inflammatory response, leads to more than 30 million infected patients and results in high morbidity worldwide every year. Currently, no efficient approaches have been developed for sepsis therapy due to antimicrobial resistance and inflammatory storm. Here, we report macrophages loaded with aggregated carbon dots (ACDs) in the lysosome, termed MCDs, to treat sepsis in immunosuppressive mice. The ACDs are constructed by negative CDs and amine‐abundant polyethyleneimine (PEI), enabling them to bear the strong antibacterial ability and enhanced photoluminescent efficacy. The ACDs are specifically located in the macrophage lysosomes, efficiently enhancing the multidrug‐resistant bacteria‐killing ability of MCDs. More importantly, the MCDs possess superior anti‐inflammatory effects such as reducing the number of pro‐inflammatory (M1) and stimulating anti‐inflammatory (M2) macrophages. These effects upregulate the inflammatory cytokines (TNF‐α, IL‐1β, IL‐4, and IL‐10), ultimately resulting in increased sepsis survival. Our work provides an intelligent approach to overcoming multidrug‐resistant bacteria‐induced infection from sepsis patients and paves a new avenue on employing nanoparticle‐loaded cells for combating inflammation‐related infection.
