Selekcija i Semenarstvo (Jan 2006)
Chemical mutagenesis in head cabbage (Brassica oleracea var.capitata L.): III effect of ethyl methane sulphonate on mycosporogenesis and pollen fertility
Abstract
The aim of present investigation was to study the effect of different ethyl methane sulfonate (EMS) concentrations (0.5, 0.6 and 0.7%) on microsporogenesis and on the expression of pollen fertility in M1 plants, obtained from treated cabbage seeds (variety "Ditmarsko"). The investigation was conducted to identify particular stages of male sterility expression at cytological level. It was established that the increase of EMS concentration resulted in decrease of pollen fertility values and in increase of the percentage of plants with low level of this trait. Cytological analysis in plants with 10.0 % pollen fertility showed, that EMS has a negative effect on the homologous chromosome pairing (early stages of PMC meiosis) and on chromosomes correct division and migration to the spindle poles (second metaphase - anaphase). As a result of this the microsporogenesis runs with disturbances and formation of non - reduced and non - balanced gametes.
