Nanoproteomic Approach for Isolation and Identification of Potential Biomarkers in Human Urine from Adults with Normal Weight, Overweight and Obesity
Sergio G. Hernandez-Leon,
Jose Andre-i Sarabia Sainz,
Gabriela Ramos-Clamont Montfort,
José Ángel Huerta-Ocampo,
Martha Nydia Ballesteros,
Ana M. Guzman-Partida,
María del Refugio Robles-Burgueño,
Luz Vazquez-Moreno
Affiliations
Sergio G. Hernandez-Leon
Centro de Investigación en Alimentación y Desarrollo A.C., Carretera Gustavo Enrique Astiazarán Rosas, No. 46, col. La Victoria, Hermosillo, Sonora 83304, Mexico
Jose Andre-i Sarabia Sainz
Departamento de Investigación en Física, Universidad de Sonora, Blvd. Luis Encinas y Rosales S/N, Col. Centro, Hermosillo, Sonora 83190, Mexico
Gabriela Ramos-Clamont Montfort
Centro de Investigación en Alimentación y Desarrollo A.C., Carretera Gustavo Enrique Astiazarán Rosas, No. 46, col. La Victoria, Hermosillo, Sonora 83304, Mexico
José Ángel Huerta-Ocampo
CONACyT-Centro de Investigación en Alimentación y Desarrollo A.C., Carretera Gustavo Enrique Astiazarán Rosas, No. 46, col. La Victoria, Hermosillo, Sonora 83304, Mexico
Martha Nydia Ballesteros
Centro de Investigación en Alimentación y Desarrollo A.C., Carretera Gustavo Enrique Astiazarán Rosas, No. 46, col. La Victoria, Hermosillo, Sonora 83304, Mexico
Ana M. Guzman-Partida
Centro de Investigación en Alimentación y Desarrollo A.C., Carretera Gustavo Enrique Astiazarán Rosas, No. 46, col. La Victoria, Hermosillo, Sonora 83304, Mexico
María del Refugio Robles-Burgueño
Centro de Investigación en Alimentación y Desarrollo A.C., Carretera Gustavo Enrique Astiazarán Rosas, No. 46, col. La Victoria, Hermosillo, Sonora 83304, Mexico
Luz Vazquez-Moreno
Centro de Investigación en Alimentación y Desarrollo A.C., Carretera Gustavo Enrique Astiazarán Rosas, No. 46, col. La Victoria, Hermosillo, Sonora 83304, Mexico
In this work, previously synthesized and characterized core-shell silica nanoparticles (FCSNP) functionalized with immobilized molecular bait, Cibacron blue, and a porous polymeric bis-acrylamide shell were incubated with pooled urine samples from adult women or men with normal weight, overweight or obesity for the isolation of potential biomarkers. A total of 30 individuals (15 woman and 15 men) were included. FCSNP allowed the capture of a variety of low molecular weight (LMW) proteins as evidenced by mass spectrometry (MS) and the exclusion of high molecular weight (HMW) proteins (>34 kDa) as demonstrated by SDS-PAGE and 2D SDS-PAGE. A total of 36 proteins were successfully identified by MS and homology database searching against the Homo sapiens subset of the Swiss-Prot database. Identified proteins were grouped into different clusters according to their abundance patterns. Four proteins were found only in women and five only in men, whereas 27 proteins were in urine from both genders with different abundance patterns. Based on these results, this new approach represents an alternative tool for isolation and identification of urinary biomarkers.
