UBE2I stimulates female gonadal differentiation in chicken (Gallus gallus) embryos

Kai JIN; Jing ZHOU; Qi-sheng ZUO; Jian-cheng LI; SONG Jiuzhou; Ya-ni ZHANG; Guo-bing CHANG; Guo-hong CHEN; Bi-chun LI

Journal of Integrative Agriculture (Nov 2021)

UBE2I stimulates female gonadal differentiation in chicken (Gallus gallus) embryos

Kai JIN,
Jing ZHOU,
Qi-sheng ZUO,
Jian-cheng LI,
SONG Jiuzhou,
Ya-ni ZHANG,
Guo-bing CHANG,
Guo-hong CHEN,
Bi-chun LI

Affiliations

Kai JIN: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, P.R.China
Jing ZHOU: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, P.R.China
Qi-sheng ZUO: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, P.R.China
Jian-cheng LI: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, P.R.China
SONG Jiuzhou: Animal & Avian Sciences, University of Maryland, College Park, MD, 20741, USA
Ya-ni ZHANG: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, P.R.China
Guo-bing CHANG: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, P.R.China
Guo-hong CHEN: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, P.R.China
Bi-chun LI: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, P.R.China; Correspondence LI Bichun, Tel: +86-514-87977207, Fax: +86-514-87350440

Journal volume & issue: Vol. 20, no. 11
pp. 2986 – 2994

Abstract

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Without known analogous sex-determining factors like SRY (sex determining region Y) in mammals, the chicken (Gallus gallus) sex determination mechanism still remains unclear, which highly restricts the biological research on chicken development and poultry single-sex reproduction. Here we not only characterized a new female-biased gene UBE2I and identified the expression pattern by qRT-PCR, but also described the functional role of UBE2I in the gonadal development of chicken embryos. Results showed that UBE2I exhibited a female-biased expression pattern in the early stage of PGCs (primordial germ cells) in embryonic gonads and robust expression in ovaries of newborn chickens. Most importantly, we successfully developed an effective method to interfere or overexpress UBE2I in chicken embryos through the intravascular injection. The qRT-PCR analysis showed that the sex-related genes (FOXL2, CYP19A1 and HINTW) in females were upregulated (P<0.05) under the overexpression of UBE2I and the sex-related genes (SOX9, DMRT1 and WT1) in females were downregulated (P<0.05) after interfering UBE2I. Furthermore, the change of UBE2I expression was associated with the level of estradiol and its receptors (AR and ESR), which suggests that UBE2I is necessary to initiate the female-specific development in chickens. In conclusion, this work demonstrates that UBE2I is a crucial sex differentiation-related gene in the embryonic development of chickens, which provides insights for further understanding the mechanism of sex determination in chickens.

Published in Journal of Integrative Agriculture

ISSN: 2095-3119 (Print); 2352-3425 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.journals.elsevier.com/journal-of-integrative-agriculture

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