Nature Communications (Feb 2023)

Antigen footprint governs activation of the B cell receptor

  • Alexey Ferapontov,
  • Marjan Omer,
  • Isabelle Baudrexel,
  • Jesper Sejrup Nielsen,
  • Daniel Miotto Dupont,
  • Kristian Juul-Madsen,
  • Philipp Steen,
  • Alexandra S. Eklund,
  • Steffen Thiel,
  • Thomas Vorup-Jensen,
  • Ralf Jungmann,
  • Jørgen Kjems,
  • Søren Egedal Degn

DOI
https://doi.org/10.1038/s41467-023-36672-0
Journal volume & issue
Vol. 14, no. 1
pp. 1 – 20

Abstract

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The antigen-B-cell-receptor interaction is the driving force of terminal B cell development that spans from B cell activation to antibody secreting plasma cells. Here authors determine, using DNA-PAINT super-resolution microscopy, how antigen affinity and valency define antigen binding to BCR in an in vitro system allowing precision control of these parameters.