Focused size selection of cell-free DNA samples for liquid biopsy applications that rely on next-generation sequencing

Abstract

Genomic analysis of circulating, cell-free DNA (cfDNA) is being used extensively for molecular diagnostics. Many approaches rely on the construction of cfDNA genomic libraries, targeted retrieval of specific genomic regions and analysis by next-generation DNA sequencing. Several steps during sample preparation require isolation of DNA fragments within a particular size range. In this Benchmark article, two related methods for size-selective DNA fragment enrichment are described.

Keywords

• cell-free DNA
• DNA purification
• liquid biopsy
• next-generation sequencing
• SPRI beads