PLoS ONE (Jan 2015)

Polyanionic Carboxyethyl Peptide Nucleic Acids (ce-PNAs): Synthesis and DNA Binding.

  • Yuliya Kirillova,
  • Nataliya Boyarskaya,
  • Andrey Dezhenkov,
  • Mariya Tankevich,
  • Ivan Prokhorov,
  • Anna Varizhuk,
  • Sergei Eremin,
  • Dmitry Esipov,
  • Igor Smirnov,
  • Galina Pozmogova

DOI
https://doi.org/10.1371/journal.pone.0140468
Journal volume & issue
Vol. 10, no. 10
p. e0140468

Abstract

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New polyanionic modifications of polyamide nucleic acid mimics were obtained. Thymine decamers were synthesized from respective chiral α- and γ-monomers, and their enantiomeric purity was assessed. Here, we present the decamer synthesis, purification and characterization by MALDI-TOF mass spectrometry and an investigation of the hybridization properties of the decamers. We show that the modified γ-S-carboxyethyl-T10 PNA forms a stable triplex with polyadenine DNA.