Toxicopathological Impacts Of Chlorpyrifos On Sperm Qualities And Testicular Tissue Alterations And Their Modulation With Vitamin E And Zinc In Male Albino Rats

Abstract

This study was carried out on 30 mature male albino rats (2 months old, weighed about 130 g) in 3 groups of ten rats for each one in ARC. Group I, kept as control. Group II, daily intubated with CPF, at a dose level of 10.6 mg/kg BW in corn oil. Group III, daily intubated with CPF at a dose level of 10.6 mg/kg BW, zinc at a dose level of 227 mg/kg BW and vitamin E at a dose level of 75 mg/kg BW for 50 days equivalent to one spermatogenic cycle in rats. At the end of the experiment, the rats were sacrificed to take blood, semen and tissue specimens. Histopathologically, the testes and epididymis showed moderate degeneration and thickening of the tubular membrane with interstitial oedema in the CPF intoxicated group. These changes were ameliorated in the rats of group III. The semen analysis is aggressively badly affected in the CPF intoxicated group in comparison with the control group and improved in rats of group III. Testosterone hormone levels were assayed with ELISA technique as 3.871±0.31 ng/ml, 1.112±0.82 ng/ml, and 2.503±0.25 ng/ml in control, CPF, CPF plus vitamin E and Zn groups, respectively. Moreover, the DNA integrity of spermatozoa in the CPF group was severely affected in comparison with either the control group and group III.

Keywords

• antioxidants
• chlorpyrifos
• histopathology
• semen
• spermatogenic cycle