Frontiers in Immunology (Feb 2022)
Identification of Distinct Immune Cell Subsets Associated With Asymptomatic Infection, Disease Severity, and Viral Persistence in COVID-19 Patients
- Xiaorui Wang,
- Xiaorui Wang,
- Han Bai,
- Han Bai,
- Junpeng Ma,
- Junpeng Ma,
- Hongyu Qin,
- Hongyu Qin,
- Qiqi Zeng,
- Qiqi Zeng,
- Fang Hu,
- Fang Hu,
- Tingting Jiang,
- Weikang Mao,
- Yang Zhao,
- Xiaobei Chen,
- Xin Qi,
- Xin Qi,
- Mengyang Li,
- Mengyang Li,
- Jiao Xu,
- Jiao Xu,
- Jingcan Hao,
- Yankui Wang,
- Xi Ding,
- Yuanrui Liu,
- Tianlong Huang,
- Chao Fang,
- Changli Ge,
- Dong Li,
- Ke Hu,
- Xianwen Ren,
- Baojun Zhang,
- Binghong Zhang,
- Bingyin Shi,
- Chengsheng Zhang,
- Chengsheng Zhang,
- Chengsheng Zhang,
- Chengsheng Zhang
Affiliations
- Xiaorui Wang
- Precision Medicine Center, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Xiaorui Wang
- The MED-X Institute, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Han Bai
- Precision Medicine Center, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Han Bai
- The MED-X Institute, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Junpeng Ma
- Precision Medicine Center, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Junpeng Ma
- The MED-X Institute, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Hongyu Qin
- Precision Medicine Center, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Hongyu Qin
- The MED-X Institute, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Qiqi Zeng
- Precision Medicine Center, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Qiqi Zeng
- The MED-X Institute, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Fang Hu
- Precision Medicine Center, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Fang Hu
- The MED-X Institute, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Tingting Jiang
- LC-Bio Technologies, Co., Ltd., Hangzhou, China
- Weikang Mao
- LC-Bio Technologies, Co., Ltd., Hangzhou, China
- Yang Zhao
- Department of Respiratory and Critical Care Medicine, Renmin Hospital of Wuhan University, Wuhan, China
- Xiaobei Chen
- Department of Infectious Diseases, The Renmin Hospital of Wuhan University, Wuhan, China
- Xin Qi
- Precision Medicine Center, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Xin Qi
- The MED-X Institute, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Mengyang Li
- Precision Medicine Center, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Mengyang Li
- Department of Endocrinology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Jiao Xu
- Precision Medicine Center, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Jiao Xu
- The MED-X Institute, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Jingcan Hao
- Cancer Center, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Yankui Wang
- Dialysis Center, The Renmin Hospital of Wuhan University, Wuhan, China
- Xi Ding
- Department of Endocrinology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Yuanrui Liu
- LC-Bio Technologies, Co., Ltd., Hangzhou, China
- Tianlong Huang
- LC-Bio Technologies, Co., Ltd., Hangzhou, China
- Chao Fang
- LC-Bio Technologies, Co., Ltd., Hangzhou, China
- Changli Ge
- LC-Bio Technologies, Co., Ltd., Hangzhou, China
- Dong Li
- Department of Clinical Laboratory, The Renmin Hospital of Wuhan University, Wuhan, China
- Ke Hu
- Department of Respiratory and Critical Care Medicine, Renmin Hospital of Wuhan University, Wuhan, China
- Xianwen Ren
- 0Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing, China
- Baojun Zhang
- 1Department of Pathogenic Microbiology and Immunology, School of Basic Medical Sciences, Xi’an Jiaotong University, Xi’an, China
- Binghong Zhang
- 2The Renmin Hospital of Wuhan University, Wuhan, China
- Bingyin Shi
- Department of Endocrinology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Chengsheng Zhang
- Precision Medicine Center, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Chengsheng Zhang
- The MED-X Institute, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Chengsheng Zhang
- Cancer Center, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Chengsheng Zhang
- 3The Jackson Laboratory for Genomic Medicine, Farmington, CT, United States
- DOI
- https://doi.org/10.3389/fimmu.2022.812514
- Journal volume & issue
-
Vol. 13
Abstract
The cell-mediated protective and pathogenic immune responses to SARS-CoV-2 infection remain largely elusive. Here we identified 76 distinct cell subsets in the PBMC samples that were associated with various clinical presentations of COVID-19 using scRNA-seq technology coupled with a deep and comprehensive analysis of unique cell surface markers and differentially expressed genes. We revealed that (TRAV1-2+CD8+)MAIT cells and (NCAM1hiCD160+)NK cells significantly enriched in the asymptomatic subjects whereas (LAG3+CD160+CD8+)NKT cells increased in the symptomatic patients. We also observed that (CD68-CSF1R-IL1BhiCD14+)classical monocytes were positively correlated with the disease severity. Moreover, (CD33-HLA-DMA-CD14+)classical monocytes and (CLEC10A-S100A9lo)pDC were associated with the viral persistence. The GO and KEGG analyses identified enriched pathways related to immune responses, inflammation, and apoptosis. These findings may enhance our understanding of the immunopathogenesis of COVID-19 and help develop novel strategies against SARS-CoV-2 infection.
Keywords
- SARS-CoV-2
- COVID-19
- asymptomatic infection
- disease severity
- viral persistence
- singlecell RNA sequencing (scRNA-seq)
