Using Fiber Photometry in Mice to Estimate Fluorescent Biosensor Levels During Sleep
Mie Andersen,
Anastasia Tsopanidou,
Tessa Radovanovic,
Viviane Compere,
Natalie Hauglund,
Maiken Nedergaard,
Celia Kjaerby
Affiliations
Mie Andersen
Center for Translational Neuromedicine, University of Copenhagen, Noerre Alle 14, 2200 Copenhagen, Denmark
Anastasia Tsopanidou
Center for Translational Neuromedicine, University of Copenhagen, Noerre Alle 14, 2200 Copenhagen, Denmark
Tessa Radovanovic
Center for Translational Neuromedicine, University of Copenhagen, Noerre Alle 14, 2200 Copenhagen, Denmark
Viviane Compere
Center for Translational Neuromedicine, University of Copenhagen, Noerre Alle 14, 2200 Copenhagen, Denmark
Natalie Hauglund
Center for Translational Neuromedicine, University of Copenhagen, Noerre Alle 14, 2200 Copenhagen, Denmark
Maiken Nedergaard
Center for Translational Neuromedicine, University of Copenhagen, Noerre Alle 14, 2200 Copenhagen, DenmarkCenter for Translational Neuromedicine, Department of Neurosurgery, University of Rochester Medical Center, Rochester, NY, USA
Celia Kjaerby
Center for Translational Neuromedicine, University of Copenhagen, Noerre Alle 14, 2200 Copenhagen, Denmark
Sleep is not homogenous but contains a highly diverse microstructural composition influenced by neuromodulators. Prior methods used to measure neuromodulator levels in vivo have been limited by low time resolution or technical difficulties in achieving recordings in a freely moving setting, which is essential for natural sleep. In this protocol, we demonstrate the combination of electroencephalographic (EEG)/electromyographic (EMG) recordings with fiber photometric measurements of fluorescent biosensors for neuromodulators in freely moving mice. This allows for real-time assessment of extracellular neuromodulator levels during distinct phases of sleep with a high temporal resolution.
