Artemisinin-loaded mesoporous silica nanoparticles/electrospun poly(lactic-co-glycolic acid) composite nanofibers for enhanced anticancer efficiency in breast cancer cells

Abstract

Abstract Purpose Antihyperglycemic drug artemisinin (Art) has recently gained attention as a potential anticancer treatment. In this study, the poly(lactic-co-glycolic acid) (PLGA) polymer was used to create Art-containing nanofibers (NFs) using the electrospinning technique. Methods The morphological characteristics, rate of degradation, and drug release profile of the NFs were described. In addition, we examined both the cytotoxic effects and internalization of reactive oxygen species (ROS), as well as the expression levels of apoptotic genes following the treatment of SK-BR-3 breast cancer cells with Art and Art-loaded PLGA nanofibers. Results The bead-free, smooth surface, and randomly aligned electrospun NFs released the medication quickly at first and then steadily for more than 2 weeks. They also showed a rather steady rate of deterioration over the course of 24 days. After 48h, SK-BR-3 cells exposed to ART-loaded NFs shown a substantial cytotoxicity compared to free Art. Additionally, Art-loaded NFs effectively increased intracellular ROS levels, inducing death in cancer cells. Gene expression studies further demonstrated the ability of the produced Art-loaded NFs to significantly modify Bax and Bcl-2 levels as well as activate caspases-3 and P53 compared to free Art. Conclusion Overall, the results demonstrated the Art-loaded PLGA nanofibers anticancer effectiveness, indicating that it may be employed as an implantable drug delivery system to decrease breast cancer recurrence following surgical resection.

Keywords

• Artemisinin
• Electrospinning
• Nanofiber
• PLGA
• Breast cancer