Isolation of Moderately Halophilic Bacteria in Saline Environments of Sonora State Searching for Proteolytic Hydrolases

Abstract

The aim of the study was to isolate moderately halophilic bacteria that produce proteolytic enzymes with industrial biotechnological value. Screening of halophiles from various saline habitats, led to the isolation of 210 moderately halophilic bacteria producing industrially important hydrolases; such as proteases, which are enzymes that hydrolyze the peptide bonds of proteins, represent one of the three groups of industrial enzymes. The present study of halophilic bacteria, producing proteases and isolated from different saline soils of Sonora State, was divided in two parts: the first part included screening of moderately halophiles bacteria collected from various regions, while the second part consisted of enzyme production by fermentation in liquid medium in flask. Three strains of bacteria with potential proteolytic activity (BLRMAM1066, BLCLAM1064, PPSE3AM1053) were selected to continue the electrophoresis and zymogram tests, obtaining molecular weights from 19 to 193 kDa. One of the a priori objectives of this research is to have a collection of halophilic bacteria with high proteolytic activity

Keywords

• isolation
• screening
• saline areas
• moderate halophiles
• sds-page
• proteases