Three-Dimensional Bioprinting of Functional Skeletal Muscle Tissue Using Gelatin Methacryloyl-Alginate Bioinks

Rasoul Seyedmahmoud; Betül Çelebi-Saltik; Natan Barros; Rohollah Nasiri; Ethan Banton; Amir Shamloo; Nureddin Ashammakhi; Mehmet  Remzi Dokmeci; Samad Ahadian

doi:10.3390/mi10100679

Micromachines (Oct 2019)

Three-Dimensional Bioprinting of Functional Skeletal Muscle Tissue Using Gelatin Methacryloyl-Alginate Bioinks

Rasoul Seyedmahmoud,
Betül Çelebi-Saltik,
Natan Barros,
Rohollah Nasiri,
Ethan Banton,
Amir Shamloo,
Nureddin Ashammakhi,
Mehmet Remzi Dokmeci,
Samad Ahadian

Affiliations

Rasoul Seyedmahmoud: Department of Bioengineering, Samueli School of Engineering, University of California-Los Angeles, Los Angeles, CA 90095, USA
Betül Çelebi-Saltik: Department of Bioengineering, Samueli School of Engineering, University of California-Los Angeles, Los Angeles, CA 90095, USA
Natan Barros: Department of Bioengineering, Samueli School of Engineering, University of California-Los Angeles, Los Angeles, CA 90095, USA
Rohollah Nasiri: Department of Bioengineering, Samueli School of Engineering, University of California-Los Angeles, Los Angeles, CA 90095, USA
Ethan Banton: Department of Bioengineering, Samueli School of Engineering, University of California-Los Angeles, Los Angeles, CA 90095, USA
Amir Shamloo: Department of Mechanical Engineering, Sharif University of Technology, Tehran 11365-11155, Iran
Nureddin Ashammakhi: Department of Bioengineering, Samueli School of Engineering, University of California-Los Angeles, Los Angeles, CA 90095, USA
Mehmet Remzi Dokmeci: Center for Minimally Invasive Therapeutics (C-MIT), University of California-Los Angeles, Los Angeles, CA 90095, USA
Samad Ahadian: Department of Bioengineering, Samueli School of Engineering, University of California-Los Angeles, Los Angeles, CA 90095, USA

DOI: https://doi.org/10.3390/mi10100679
Journal volume & issue: Vol. 10, no. 10
p. 679

Abstract

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Skeletal muscle tissue engineering aims to fabricate tissue constructs to replace or restore diseased or injured skeletal muscle tissues in the body. Several biomaterials and microscale technologies have been used in muscle tissue engineering. However, it is still challenging to mimic the function and structure of the native muscle tissues. Three-dimensional (3D) bioprinting is a powerful tool to mimic the hierarchical structure of native tissues. Here, 3D bioprinting was used to fabricate tissue constructs using gelatin methacryloyl (GelMA)-alginate bioinks. Mechanical and rheological properties of GelMA-alginate hydrogels were characterized. C2C12 myoblasts at the density 8 × 106 cells/mL were used as the cell model. The effects of alginate concentration (0, 6, and 8% (w/v)) and crosslinking mechanism (UV crosslinking or ionic crosslinking with UV crosslinking) on printability, cell viability, proliferation, and differentiation of bioinks were studied. The results showed that 10% (w/v) GelMA-8% (w/v) alginate crosslinked using UV light and 0.1 M CaCl2 provided the optimum niche to induce muscle tissue formation compared to other hydrogel compositions. Furthermore, metabolic activity of cells in GelMA bioinks was improved by addition of oxygen-generating particles to the bioinks. It is hoped that such bioprinted muscle tissues may find wide applications in drug screening and tissue regeneration.

Published in Micromachines

ISSN: 2072-666X (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Mechanical engineering and machinery
Website: https://www.mdpi.com/journal/micromachines

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