eLife (Jul 2018)

Dual leucine zipper kinase is required for mechanical allodynia and microgliosis after nerve injury

  • Josette J Wlaschin,
  • Jacob M Gluski,
  • Eileen Nguyen,
  • Hanna Silberberg,
  • James H Thompson,
  • Alexander T Chesler,
  • Claire E Le Pichon

DOI
https://doi.org/10.7554/eLife.33910
Journal volume & issue
Vol. 7

Abstract

Read online

Neuropathic pain resulting from nerve injury can become persistent and difficult to treat but the molecular signaling responsible for its development remains poorly described. Here, we identify the neuronal stress sensor dual leucine zipper kinase (DLK; Map3k12) as a key molecule controlling the maladaptive pathways that lead to pain following injury. Genetic or pharmacological inhibition of DLK reduces mechanical hypersensitivity in a mouse model of neuropathic pain. Furthermore, DLK inhibition also prevents the spinal cord microgliosis that results from nerve injury and arises distant from the injury site. These striking phenotypes result from the control by DLK of a transcriptional program in somatosensory neurons regulating the expression of numerous genes implicated in pain pathogenesis, including the immune gene Csf1. Thus, activation of DLK is an early event, or even the master regulator, controlling a wide variety of pathways downstream of nerve injury that ultimately lead to chronic pain.

Keywords