PLoS ONE (Jan 2013)

The mechanism of effective electroacupuncture on T cell response in rats with experimental autoimmune encephalomyelitis.

  • Yumei Liu,
  • Hongwei Wang,
  • Xinyue Wang,
  • Lili Mu,
  • Qingfei Kong,
  • Dandan Wang,
  • Jinghua Wang,
  • Yao Zhang,
  • Jinfeng Yang,
  • Mingyan Zhou,
  • Guangyou Wang,
  • Bo Sun,
  • Hulun Li

DOI
https://doi.org/10.1371/journal.pone.0051573
Journal volume & issue
Vol. 8, no. 1
p. e51573

Abstract

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Previously, we demonstrated that electroacupuncture (EA) decreased lymphocyte infiltration into the spinal cords of rats presenting with experimental autoimmune encephalomyelitis (EAE), a disease model used in the study of multiple sclerosis (MS). The aim of this study was to characterize the effects of EA on the EAE. Female Lewis rats were divided into either CFA, EAE, EA, or injection with naloxone after electroacupuncture (NAL) groups. Electroacupuncture was administered every day for 21 days. To evaluate proliferation and apoptosis, lymphocytes from rats presenting with EAE were collected and cultured with β-endorphin. Immunohistochemisty, flow cytometry and radio-immunity methods were applied to detect the expression of β-endorphin. Results presented in this report demonstrate that the beneficial anti-inflammatory effects of EA on EAE were related to β-endorphin production that balances the Thl/Th2 and Th17/Treg responses. These results suggest that β-endorphin could be an important component in the development of EA-based therapies used for the treatment of EAE.