Identification and functional characterization of a short-type peptidoglycan recognition protein, PGRP-S in the orange-spotted grouper, Epinephelus coioides

Jing Hou; Zhen Gan; Shannan Chen; Jun Cheng; Yishan Lu; Bei Huang; Pin Nie; Liqun Xia

Aquaculture Reports (Jul 2021)

Identification and functional characterization of a short-type peptidoglycan recognition protein, PGRP-S in the orange-spotted grouper, Epinephelus coioides

Jing Hou,
Zhen Gan,
Shannan Chen,
Jun Cheng,
Yishan Lu,
Bei Huang,
Pin Nie,
Liqun Xia

Affiliations

Jing Hou: Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; College of Fishery, Guangdong Ocean University, Zhanjiang 524025, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment and Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen 518120, China; Shenzhen Dapeng New District Science and Technology Innovation Service Center, Shenzhen 518120, China
Zhen Gan: Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; College of Fishery, Guangdong Ocean University, Zhanjiang 524025, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment and Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen 518120, China; Shenzhen Dapeng New District Science and Technology Innovation Service Center, Shenzhen 518120, China
Shannan Chen: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
Jun Cheng: Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; College of Fishery, Guangdong Ocean University, Zhanjiang 524025, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment and Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen 518120, China
Yishan Lu: Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; College of Fishery, Guangdong Ocean University, Zhanjiang 524025, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment and Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen 518120, China
Bei Huang: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
Pin Nie: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Corresponding author at: Institute of Hydrobiology, Chinese Academy of Sciences, No. 7 of South Donghu Road, Wuchang District, Wuhan, Hubei Province, 430072, China.
Liqun Xia: Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; College of Fishery, Guangdong Ocean University, Zhanjiang 524025, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment and Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen 518120, China; Corresponding author at: Shenzhen Institute of Guangdong Ocean University, No. 3 of Binhai 2nd Road, Dapeng New District, Shenzhen, Guangdong Province, 518120, China.

Journal volume & issue: Vol. 20
p. 100739

Abstract

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Peptidoglycan recognition proteins (PGRPs) are a family of pattern recognition receptors (PRRs) mediating the recognition of peptidoglycan. In the present study, a short-type PGRP gene named Eco-PGRP-S was identified in the orange-spotted grouper (Epinephelus coioides), a major commercial marine fish cultured in Asia. Eco-PGRP-S gene was constitutively expressed in all tissues examined, with the highest expression level observed in gill and skin. As a typical PRR, Eco-PGRP-S is inducible following the stimulation of peptidoglycan, and has an ability to bind peptidoglycan. Importantly, Eco-PGRP-S has been proven to have Zn2+-dependent amidase activity and direct antibacterial activity against Vibrio Harveri and Edwardsiella tarda. In addition, Eco-PGRP-S is mainly localized in cytoplasm and nucleus, and is able to significantly trigger the activation of NF-κB signalling. The present research thus contributes to a better understanding of the molecular mechanism of antibacterial defense and the containment of infectious bacterial diseases in grouper.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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