PharmacoSTORM nanoscale pharmacology reveals cariprazine binding on Islands of Calleja granule cells

Susanne Prokop; Péter Ábrányi-Balogh; Benjámin Barti; Márton Vámosi; Miklós Zöldi; László Barna; Gabriella M. Urbán; András Dávid Tóth; Barna Dudok; Attila Egyed; Hui Deng; Gian Marco Leggio; László Hunyady; Mario van der Stelt; György M. Keserű; István Katona

doi:10.1038/s41467-021-26757-z

Nature Communications (Nov 2021)

PharmacoSTORM nanoscale pharmacology reveals cariprazine binding on Islands of Calleja granule cells

Susanne Prokop,
Péter Ábrányi-Balogh,
Benjámin Barti,
Márton Vámosi,
Miklós Zöldi,
László Barna,
Gabriella M. Urbán,
András Dávid Tóth,
Barna Dudok,
Attila Egyed,
Hui Deng,
Gian Marco Leggio,
László Hunyady,
Mario van der Stelt,
György M. Keserű,
István Katona

Affiliations

Susanne Prokop: Momentum Laboratory of Molecular Neurobiology, Institute of Experimental Medicine
Péter Ábrányi-Balogh: Medicinal Chemistry Research Group, Research Centre for Natural Sciences
Benjámin Barti: Momentum Laboratory of Molecular Neurobiology, Institute of Experimental Medicine
Márton Vámosi: Momentum Laboratory of Molecular Neurobiology, Institute of Experimental Medicine
Miklós Zöldi: Momentum Laboratory of Molecular Neurobiology, Institute of Experimental Medicine
László Barna: Nikon Center of Excellence for Neuronal Imaging, Institute of Experimental Medicine
Gabriella M. Urbán: Momentum Laboratory of Molecular Neurobiology, Institute of Experimental Medicine
András Dávid Tóth: Department of Physiology, Faculty of Medicine, Semmelweis University
Barna Dudok: Momentum Laboratory of Molecular Neurobiology, Institute of Experimental Medicine
Attila Egyed: Medicinal Chemistry Research Group, Research Centre for Natural Sciences
Hui Deng: Department of Molecular Physiology, Leiden Institute of Chemistry, Leiden University & Oncode Institute
Gian Marco Leggio: Department of Biomedical and Biotechnological Sciences, Section of Pharmacology, University of Catania
László Hunyady: Department of Physiology, Faculty of Medicine, Semmelweis University
Mario van der Stelt: Department of Molecular Physiology, Leiden Institute of Chemistry, Leiden University & Oncode Institute
György M. Keserű: Medicinal Chemistry Research Group, Research Centre for Natural Sciences
István Katona: Momentum Laboratory of Molecular Neurobiology, Institute of Experimental Medicine

DOI: https://doi.org/10.1038/s41467-021-26757-z
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 19

Abstract

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The authors introduce PharmacoSTORM single-molecule imaging that uses fluorescent ligands and immunolabeling for cellular and subcellular nanoscale molecular pharmacology. They demonstrate its capabilities by visualizing cariprazine binding to D3 dopamine receptors on Islands of Calleja granule cell axons.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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