Payload distribution and capacity of mRNA lipid nanoparticles

Sixuan Li; Yizong Hu; Andrew Li; Jinghan Lin; Kuangwen Hsieh; Zachary Schneiderman; Pengfei Zhang; Yining Zhu; Chenhu Qiu; Efrosini Kokkoli; Tza-Huei Wang; Hai-Quan Mao

doi:10.1038/s41467-022-33157-4

Nature Communications (Sep 2022)

Payload distribution and capacity of mRNA lipid nanoparticles

Sixuan Li,
Yizong Hu,
Andrew Li,
Jinghan Lin,
Kuangwen Hsieh,
Zachary Schneiderman,
Pengfei Zhang,
Yining Zhu,
Chenhu Qiu,
Efrosini Kokkoli,
Tza-Huei Wang,
Hai-Quan Mao

Affiliations

Sixuan Li: Department of Mechanical Engineering, Johns Hopkins University
Yizong Hu: Institute for NanoBioTechnology, Johns Hopkins University
Andrew Li: Department of Biomedical Engineering, Johns Hopkins University School of Medicine
Jinghan Lin: Institute for NanoBioTechnology, Johns Hopkins University
Kuangwen Hsieh: Department of Mechanical Engineering, Johns Hopkins University
Zachary Schneiderman: Institute for NanoBioTechnology, Johns Hopkins University
Pengfei Zhang: Department of Biomedical Engineering, Johns Hopkins University School of Medicine
Yining Zhu: Institute for NanoBioTechnology, Johns Hopkins University
Chenhu Qiu: Institute for NanoBioTechnology, Johns Hopkins University
Efrosini Kokkoli: Institute for NanoBioTechnology, Johns Hopkins University
Tza-Huei Wang: Department of Mechanical Engineering, Johns Hopkins University
Hai-Quan Mao: Institute for NanoBioTechnology, Johns Hopkins University

DOI: https://doi.org/10.1038/s41467-022-33157-4
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

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Lipid nanoparticles (LNPs) are effective vehicles to deliver mRNA vaccines and therapeutics but assessing the mRNA packaging characteristics in LNPs is challenging. Here, the authors report that mRNA and lipid contents in LNP formulations can be quantitatively examined by multi-laser cylindrical illumination confocal spectroscopy at the single-nanoparticle level.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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