ArfB can displace mRNA to rescue stalled ribosomes

Christine E. Carbone; Gabriel Demo; Rohini Madireddy; Egor Svidritskiy; Andrei A. Korostelev

doi:10.1038/s41467-020-19370-z

Nature Communications (Nov 2020)

ArfB can displace mRNA to rescue stalled ribosomes

Christine E. Carbone,
Gabriel Demo,
Rohini Madireddy,
Egor Svidritskiy,
Andrei A. Korostelev

Affiliations

Christine E. Carbone: RNA Therapeutics Institute, Department of Biochemistry and Molecular Pharmacology, UMass Medical School
Gabriel Demo: RNA Therapeutics Institute, Department of Biochemistry and Molecular Pharmacology, UMass Medical School
Rohini Madireddy: RNA Therapeutics Institute, Department of Biochemistry and Molecular Pharmacology, UMass Medical School
Egor Svidritskiy: RNA Therapeutics Institute, Department of Biochemistry and Molecular Pharmacology, UMass Medical School
Andrei A. Korostelev: RNA Therapeutics Institute, Department of Biochemistry and Molecular Pharmacology, UMass Medical School

DOI: https://doi.org/10.1038/s41467-020-19370-z
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 9

Abstract

Read online

Alternative rescue factor B (ArfB) is an enzyme that releases peptides from stalled ribosomes to allow ribosome recycling. Here the authors carry-out cryo-EM analyses of 70S ribosomes complexed with ArfB on either a short or longer mRNA to reveal distinct modes of ArfB function.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal